| Rabbit Anti-MTA1 antibody |
| 产品应用（已验证） |
WB,IHC |
| 产品应用（可尝试） |
IF,ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500, |
| 研究领域 |
肿瘤,细胞生物,表观遗传学, |
| 标签 |
Array |
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Tissue/cell: transplanted tumor of cervical carcinoma in nude mice; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-MTA1 Polyclonal Antibody, Unconjugated(bs-1412R) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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Tissue/cell: human cervical carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-MTA1 Polyclonal Antibody, Unconjugated(bs-1412R) 1:600, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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Sample: Lung(Mouse) Lysate at 30 ug
Primary: Anti- MTA1 (bs-1412R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/10000 dilution
Predicted band size: 81 kD
Observed band size: 85 kD
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Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MTA1) Polyclonal Antibody, Unconjugated (bs-1412R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MTA1) Polyclonal Antibody, Unconjugated (bs-1412R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
RRID：AB_10855635
产品名称：Rabbit Anti-MTA1 antibody
别名： Metastasis-associated 1; Metastasis associated 1; Metastasis associated gene 1; Metastasis associated gene 1 protein; Metastasis associated protein; Metastasis associated protein MTA 1; Metastasis associated protein MTA1; MTA 1; MTA1_HUMAN.
中文名称：肿瘤转移相关蛋白1抗体
英文名称：Rabbit Anti-MTA1 antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞核,细胞浆
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human MTA1
抗原表位：601-715/715
SWISS：Q13330
Gene ID ：9112
Human Gene ID：9112
This protein was identified in metastatic cells, specifically, mammary adenocarcinoma cell lines. Expression of this protein has been correlated with the metastatic potential of at least two types of carcinomas although it is also expressed in many normal tissues. The role it plays in metastasis is unclear. It was initially thought to be the 70 kDa component of a nucleosome remodeling deacetylase complex, NuRD, but it is more likely that this component is a different but very similar protein. These two proteins are so closely related, though, that they share the same types of domains. These domains include two DNA binding domains, a dimerization domain, and a domain commonly found in proteins that methylate DNA. The profile and activity of this protein suggests that it is involved in regulating transcription and that this may be accomplished by chromatin remodeling.
Function：May be involved in the regulation of gene expression by covalent modification of histone proteins. Isoform Long is a corepressor of estrogen receptor (ER). Isoform Short binds to ER and sequesters it in the cytoplasm and enhances non-genomic responses of
Subunit：Component of the nucleosome-remodeling and histone-deacetylase multiprotein complex (NuRD). Interacts with HDAC1 and ITGB3BP/CENPR. Binds to CSNK1G2 in the cytoplasm.
Subcellular Location：Cytoplasm and Nucleus.
Tissue Specificity：Widely expressed. High expression in brain, ovaries, adrenal glands and virgin mammary glands. Higher in tumors than in adjacent normal tissue from the same individual.
Similarity：Contains 1 BAH domain.
Contains 1 ELM2 domain.
Contains 1 GATA-type zinc finger.
Contains 1 SANT domain.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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