| Rabbit Anti-phospho-PP2A alpha+beta (Tyr307) antibody |
| 反应物种（预测） |
Mouse,Chicken,Dog,Pig,Cow |
| 产品应用（已验证） |
WB,IHC |
| 产品应用（可尝试） |
IF,ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500, |
| 研究领域 |
肿瘤,心血管,细胞生物,免疫学,神经生物学,信号转导,干细胞,细胞周期蛋白,转录调节因子,激酶和磷酸酶,表观遗传学, |
| 标签 |
Array |
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Paraformaldehyde-fixed, paraffin embedded (Rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-PP2A alpha+beta(Tyr307)) Polyclonal Antibody, Unconjugated (bs-6419R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-PP2A alpha+beta(Tyr307)) Polyclonal Antibody, Unconjugated (bs-6419R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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Sample:
Hela(Human) Cell Lysate at 30 ug
Primary: Anti-phospho-PP2A alpha+beta(Tyr307) (bs-6419R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kD
Observed band size: 34 kD
RRID：RRID
产品名称：Rabbit Anti-phospho-PP2A alpha+beta (Tyr307) antibody
别名： p-PP2A-Cα/β; PP2A alpha (phospho Y307); p-PP2A alpha(Y307); PP2A A; PP2A alpha; PP2A B; PP2A beta; PP2Ac; PP2C alpha; PP2C beta; PP2CA; PP2CB; PPP2CA; PPP2CB; Protein phosphatase 2 (formerly 2A) catalytic subunit alpha isoform; Protein phosphatase 2 (form
中文名称：磷酸化蛋白磷酸酶2A（PP2Aα）抗体
英文名称：Rabbit Anti-phospho-PP2A alpha+beta (Tyr307) antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞核,细胞浆
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthesised phosphopeptide derived from human PPP2CA around the phosphorylation site of Tyr307
抗原表位：PD(p-Y)FL
SWISS：P67775
Gene ID ：5515
Human Gene ID：5515
This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit. [provided by RefSeq, Jul 2008].
Function：PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By sim
Subunit：PP2A consists of a common heterodimeric core enzyme, composed of PPP2CA a 36 kDa catalytic subunit (subunit C) and PPP2R1A a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that assoc
Subcellular Location：Cytoplasm. Nucleus. Chromosome, centromere. Cytoplasm, cytoskeleton, spindle pole. Note=In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles. Centromeric localization requires the presence
Post-translational modifications：Reversibly methyl esterified on Leu-309. Carboxyl methylation may play a role in holoenzyme assembly, enhancing the affinity of the PP2A core enzyme for some, but not all, regulatory subunits. It varies during the cell cycle.
Phosphorylation of either
Similarity：Belongs to the PPP phosphatase family. PP-1 subfamily.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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