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Rabbit Anti-BNIP3 antibody
多克隆  |   SKU:bs-4239R

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货号:bs-3351R
¥1280
订购号:bs-4239R
¥1098.00-2900.00
货期:现货
Rabbit Anti-BNIP3 antibody
反应物种(预测)

Mouse,Rat,Chicken,Dog,Pig,Cow,Horse

产品应用(已验证)

FCM

产品应用(可尝试)

WB,ELISA

推荐稀释比例

WB=1:500-2000,Elisa=1:5000-10000,Flow Cyt=2ug/Test,

研究领域

肿瘤,心血管,免疫学,细胞凋亡

标签

Array

  • Blank control:A431.
    Primary Antibody (green line): Rabbit Anti-BNIP3 antibody (bs-4239R)
    Dilution: 2μg /10^6 cells;
    Isotype Control Antibody (orange line): Rabbit IgG .
    Secondary Antibody : Goat anti-rabbit IgG-AF488
    Dilution: 1μg /test.
    Protocol
    The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

产品信息

RRID:AB_11089948
产品名称:Rabbit Anti-BNIP3 antibody
别名: BCL2 Adenovirus E1B 19kDa Interacting Protein 3; BCL2/adenovirus E1B 19 kDa protein interacting protein 3; BCL2/adenovirus E1B 19 kDa protein-interacting protein 3; BNIP 3; BNIP3_HUMAN; NIP 3; NIP3.
中文名称:促凋亡调节蛋白BNIP3抗体
英文名称:Rabbit Anti-BNIP3 antibody
抗体来源: Rabbit
克隆类型:多克隆
细胞定位:细胞浆,细胞膜
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

免疫原信息

免 疫 原:KLH conjugated synthetic peptide derived from human BNIP3
抗原表位:101-194/194
SWISS:Q12983
Gene ID :664
Human Gene ID:664

产品介绍

Apoptosis-inducing protein that, which can overcome BCL2 suppression. May play a role in repartitioning calcium between the two major intracellular calcium stores in association with BCL2.
Function:Apoptosis-inducing protein that can overcome BCL2 suppression. May play a role in repartitioning calcium between the two major intracellular calcium stores in association with BCL2. Involved in mitochondrial quality control via its interaction with SPATA1
Subunit:Homodimer. Binds to BCL2. Interacts with BNIP3L and ACAA2. Also can interact with adenovirus E1B 19 kDa protein or Epstein-Barr virus BHRF1. Interacts (via BH3 domain) with SPATA18 (via coiled-coil domains).
Subcellular Location:Mitochondrion. Mitochondrion membrane. Coexpression with the EIB 19-kDa protein results in a shift in NIP3 localization pattern to the nuclear envelope. Colocalizes with ACAA2 in the mitochondria.
Similarity:Belongs to the NIP3 family.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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