| Rabbit Anti-phospho-RUNX2 (Ser451) antibody |
| 反应物种（预测） |
Chicken,Dog,Pig,Cow,Horse |
| 产品应用（已验证） |
FCM |
| 产品应用（可尝试） |
ELISA |
| 推荐稀释比例 |
Elisa=1:5000-10000,Flow Cyt=1ug/Test, |
| 研究领域 |
免疫学,干细胞,转录调节因子 |
| 标签 |
Array |
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Blank control:HL-60.
Primary Antibody (green line): Rabbit Anti-phospho-RUNX2 (Ser451) antibody (bs-5685R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
RRID：AB_11073291
产品名称：Rabbit Anti-phospho-RUNX2 (Ser451) antibody
别名： Runx-2; RUNX2(phospho S451); Runt-related Transcription Factor 2; CBF alpha 1; CBFA1; CCD; CCD1; Cleidocranial dysplasia 1; Core binding factor; Core binding factor runt domain alpha subunit 1; Core binding factor subunit alpha 1; MGC120023; Oncogene AML
中文名称：磷酸化成骨特异性转录因子抗体
英文名称：Rabbit Anti-phospho-RUNX2 (Ser451) antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞核
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated Synthesised phosphopeptide derived from human RUNX2 around the phosphorylation site of Ser451
抗原表位：TS(p-S)GS
SWISS：Q13950
Gene ID ：860
Human Gene ID：860
Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters (By similarity). Inhibits MYST4-dependent transcriptional activation.
Subunit：Heterodimer of an alpha and a beta subunit. Interacts with HIVEP3 (By similarity). The alpha subunit binds DNA as a monomer and through the Runt domain. DNA-binding is increased by heterodimerization. Interacts with G22P1 (Ku70) and XRCC5 (Ku80). Interact
Subcellular Location：Nucleus.
Tissue Specificity：Specifically expressed in osteoblasts.
Similarity：Contains 1 Runt domain.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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