Rabbit Anti-ZNRF3 antibody |
反应物种(预测) |
Mouse,Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit,Sheep |
产品应用(已验证) |
WB,IHC |
产品应用(可尝试) |
ICC,IF,ELISA |
推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:50-200,ICC=1:100-500, |
研究领域 |
细胞生物,免疫学,神经生物学,锌指蛋白,表观遗传学, |
标签 |
Array |
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Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ZNRF3) Polyclonal Antibody, Unconjugated (bs-9141R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Sample:
Lane 1: Human HepG2 cell Lysates
Lane 2: Human A549 cell Lysates
Lane 3: Human U-2 OS cell Lysates
Primary: Anti-ZNRF3 (bs-9141R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 95kDa
Observed band size: 120kDa
RRID:RRID
产品名称:Rabbit Anti-ZNRF3 antibody
别名: Novel C3HC4 type Zinc finger (ring finger); RING finger protein 203; RNF203; Zinc and ring finger 3; Zinc/RING finger protein 3; Znrf3; ZNRF3_HUMAN.
中文名称:锌指蛋白3/环指蛋白3抗体
英文名称:Rabbit Anti-ZNRF3 antibody
抗体来源: Rabbit
克隆类型:多克隆
细胞定位:细胞膜
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原:KLH conjugated synthetic peptide derived from human ZNRF3
抗原表位:101-200/936
抗原细胞定位:Extracellular
SWISS:Q9ULT6
Gene ID :84133
Human Gene ID:84133
Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. The RING-type zinc finger motif is present in a number of viral and eukaryotic proteins and is made of a conserved cysteine-rich domain that is able to bind two zinc atoms. Proteins that contain this conserved domain are generally involved in the ubiquitination pathway of protein degradation. The three classes of enzymes involved in ubiquitination are the ubiquitin-activating enzymes (E1s), the ubiquitin-conjugating enzymes (E2s) and the ubiquitin-protein ligases (E3s). ZNRF3 (Zinc/RING finger protein 3), also known as RNF203 (RING finger protein 203), is a 936 amino acid single pass transmembrane protein that contains one RING-type zinc finger. Related zinc/RING finger proteins, such as ZNRF1 and ZNRF2, are E3 ubiquitin-protein ligases that are thought to be involved in the establishment and maintenance of neuronal transmission and plasticity, therefore it is likely that ZNRF3 may function in a similar manner.
Function:E3 ubiquitin-protein ligase that acts as a negative regulator of the Wnt signaling pathway by mediating the ubiquitination and subsequent degradation of Wnt receptor complex components Frizzled and LRP6. Acts on both canonical and non-canonical Wnt signal
Subunit:Interacts with LRP6, FZD4, FZD5, FZD6 and FZD8. Interacts with RSPO1; interaction promotes indirect interaction with LGR4 and membrane clearance of ZNRF3.
Subcellular Location:Cell membrane; Single-pass type I membrane protein.
Similarity:Belongs to the ZNRF3 family.
Contains 1 RING-type zinc finger.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.