Rabbit Anti-MRF/C11orf9 antibody |
反应物种(预测) |
Chicken,Dog,Pig,Cow,Horse,Rabbit,Sheep |
产品应用(已验证) |
WB,IHC |
产品应用(可尝试) |
ICC,IF,ELISA |
推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500, |
研究领域 |
细胞生物,免疫学,神经生物学,转录调节因子,表观遗传学, |
标签 |
Array |
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Sample:
Uterus (Mouse) Lysate at 40 ug
Primary: Anti- MRF/C11orf9 (bs-11191R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 124 kD
Observed band size: 124 kD
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Sample:
Lane 1: Human A549 cell lysates
Lane 2: Human HepG2 cell lysates
Lane 3: Human U2OS cell lysates
Lane 3: Human A431 cell lysates
Primary: Anti- MRF/C11orf9 (bs-11191R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 124 kDa
Observed band size: 124 kDa
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Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-MRF/C11orf9 Polyclonal Antibody, Unconjugated(bs-11191R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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Sample: Brain (Mouse) Lysate at 40 ug
Primary: Anti-MRF/C11orf9 (bs-11191R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
Predicted band size: 124 kD
Observed band size: 124 kD
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Sample:
A549(Human) Cell Lysate at 30 ug
Primary: Anti- MRF/C11orf9 (bs-11191R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 124 kD
Observed band size: 124 kD
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Sample:
Lung (Mouse) Lysate at 40 ug
Primary: Anti- MRF/C11orf9 (bs-11191R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 124 kD
Observed band size: 124 kD
RRID:RRID
产品名称:Rabbit Anti-MRF/C11orf9 antibody
别名: C11orf9; Chromosome 11 open reading frame 9; Gm1804; gm98; KIAA0954; MGC10781; MRF; MRF_HUMAN; Myelin gene regulatory factor; Ndt80.
中文名称:髓鞘基因调控因子抗体
英文名称:Rabbit Anti-MRF/C11orf9 antibody
抗体来源: Rabbit
克隆类型:多克隆
细胞定位:细胞核,细胞膜
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原:KLH conjugated synthetic peptide derived from human MRF
抗原表位:401-500/1151
SWISS:Q9Y2G1
Gene ID :745
Human Gene ID:745
C11orf9 (chromosome 11 open reading frame 9), also known as MRF (myelin gene regulatory factor), Ndt80, KIAA0954 or MGC10781, is a 1,151 amino acid single-pass membrane protein that localizes to nucleus. A member of the MRF family, C11orf9 is expressed in the ARPE-19 cell line, brainstem, uterus and, to a lesser extent, in basal ganglion and liver. C11orf9 is weakly expressed in cerebellum and retina. C11orf9 contains one NDT80 DNA-binding domain and acts as a transcription factor that is required for expression of central nervous system (CNS) myelin genes such as MBP and MOG, thereby playing a central role in oligodendrocyte maturation and CNS myelination. C11orf9 exists as two alternatively spliced isoforms and is encoded by a gene located on human chromosome 11, which consists of approximately 135 million base pairs and 1,400 genes. Chromosome 11 makes up around 4% of human genomic DNA and is considered a gene and disease association dense chromosome.
Function:Transcription regulator required for expression of central nervous system (CNS) myelin genes such as MBP and MOG, thereby playing a central role in oligodendrocyte maturation and CNS myelination. Probably acts as a transcription factor that directly binds
Subunit:Belongs to the MRF family.
Contains 1 NDT80 DNA-binding domain.
Subcellular Location:Membrane. Nucleus.
Tissue Specificity:Expressed in lung, ARPE-19 cell line, brainstem, uterus and, to a lesser extent, in basal ganglion and liver. Weakly expressed in cerebellum and retina.
Similarity:Belongs to the MRF family.
Contains 1 NDT80 DNA-binding domain.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.