| Rabbit Anti-Piwil2 antibody |
| 反应物种（预测） |
Dog,Pig,Cow,Rabbit,GuineaPig |
| 产品应用（已验证） |
WB,FCM |
| 产品应用（可尝试） |
IHC,IF,ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=0.2μg /Test,IF=1:100-500, |
| 研究领域 |
肿瘤,细胞生物,免疫学,信号转导,转录调节因子 |
| 标签 |
Array |
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Sample:
Lovo(Human) Cell Lysate at 30 ug
A549(Human) Cell Lysate at 30 ug
Primary: Anti-Piwil2 (bs-3817R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 100/110 kD
Observed band size: 110 kD
-
Sample:
Testis (Mouse) Lysate at 40 ug
Testis (Rat) Lysate at 40 ug
HepG2 (Human) Cell Lysate at 30 ug
Lovo (Human) Cell Lysate at 30 ug
293T (Human) Cell Lysate at 30 ug
A549 (Human) Cell Lysate at 30 ug
Primary: Anti-Piwil2 (bs-3817R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 100/110 kD
Observed band size: 110 kD
-
Blank control: Mouse spleen cells(fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice).
Primary Antibody:Rabbit Anti-Piwil2 antibody(bs-3817R), Dilution: 0.2μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
-
Sample: 293T Cell Lysate at 30 ug
Primary: Anti- Piwil2 (bs-3817R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 107 kD
Observed band size: 107kD
-
Sample:
testis (Mouse) Lysate at 40 ug
293T Cell (Human) Lysate at 30 ug
Primary: Anti- piwil2 (bs-3817R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 107 kD
Observed band size: 107 kD
RRID：AB_10856682
产品名称：Rabbit Anti-Piwil2 antibody
别名： HILI; Hiwi like; MILI; Miwi like; Piwi like 2; Piwi-like 2; Piwil 2; Piwil-2; Piwil2; Cancer/testis antigen 80; CT80; PIWL2_HUMAN; HILI.
中文名称：piwi样2蛋白抗体
英文名称：Rabbit Anti-Piwil2 antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞浆
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human Piwil2/Mili
抗原表位：885-973/973
SWISS：Q8TC59
Gene ID ：55124
Human Gene ID：55124
HILI is a 973 amino acid protein encoded by the human gene PIWIL2. HILI belongs to the argonaute family and contains one PAZ domain and one PIWI domain. HILI is a cytoplasmic protein that is expressed in adult testis and in most tumors. It regulates spermatogenesis and primordial germ cell production and has an essential role in meiotic differentiation of spermatocytes and in self-renewal of spermatogonial stem cells. Expression of HILI can modulate expression of genes involved in stem cell proliferation (such as PDGFR-b), in energy metabolism (such as Glut1), in cell-cell interaction (such as Integrin a6, GJA7, THY-1 and CD9), and in germ cell differentiation (such as STRA8). It may also play a role as a regulatory factor of Stat3/Bcl-xS/L/CCND1 pathway. Repression of HILI can inhibit tumor cell growth. HILI acts as an oncogene by inhibition of apoptosis and promotion of proliferation in tumors.
Function：Plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Plays an essential role in meiotic differentiation of spermatocytes, germ cell differentiation a
Subunit：Interacts with DDX4, MAEL, EIF3A, EIF4E, EIF4G, PRMT5 and WDR77. Associates with EIF4E- and EIF4G-containing m7G cap-binding complexes. Interacts (when methylated on arginine residues) with TDRD1.
Subcellular Location：Cytoplasm. Note=Present in chromatoid body. Probable component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon during meiosis.
Tissue Specificity：Expressed in adult testis and in most tumors.
Post-translational modifications：Arginine methylation by PRMT5 is required for the interaction with Tudor domain-containing protein TDRD1 and subsequent localization to the meiotic nuage, also named P granule.
Similarity：Belongs to the argonaute family. Piwi subfamily.
Contains 1 PAZ domain.
Contains 1 Piwi domain.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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