| Rabbit Anti-Phospho-SGK1 (Ser422) antibody |
| 反应物种（预测） |
Dog,Pig,Horse,Rabbit |
| 产品应用（已验证） |
WB,IHC,FCM |
| 产品应用（可尝试） |
IF,ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=2ug/Test,IF=1:100-500, |
| 研究领域 |
免疫学,发育生物学,信号转导,干细胞,激酶和磷酸酶,细胞表面分子, |
| 标签 |
Array |
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Sample:
Muscle (Mouse) Lysate at 40 ug
Primary: Anti- Phospho-SGK1 (Ser422) (bs-3396R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49 kD
Observed band size: 49 kD
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Sample:
Pancreas (Mouse) Lysate at 40 ug
Primary: Anti-Phospho-SGK1 (Ser422) (bs-3396R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49 kD
Observed band size: 49 kD
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Blank control: Mouse spleen.
Primary Antibody (green line): Rabbit Anti-SGK1 antibody (bs-3396R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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Sample:
Lane 1: Pancreas (Mouse) Lysate at 40 ug
Lane 2: Kidney (Mouse) Lysate at 40 ug
Lane 3: Cerebrum (Mouse) Lysate at 40 ug
Lane 4: Pancreas (Rat) Lysate at 40 ug
Lane 5: Cerebrum (Rat) Lysate at 40 ug
Primary: Anti-Phospho-SGK1 (Ser422) (bs-3396R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45-60 kD
Observed band size: 50 kD
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Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Phospho-SGK1(Ser422) Polyclonal Antibody, Unconjugated(bs-3396R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
RRID：AB_10856714
产品名称：Rabbit Anti-Phospho-SGK1 (Ser422) antibody
别名： SGK1(Phospho Ser422); SGK1 (Phospho S422); Serine/threonine protein kinase SGK; Serine/threonine protein kinase Sgk1; Serine/threonine-protein kinase Sgk1; Serum and glucocorticoid regulated kinase; Serum/glucocorticoid regulated kinase 1; Serum/glucocort
中文名称：磷酸化糖皮质激素调节激酶1抗体
英文名称：Rabbit Anti-Phospho-SGK1 (Ser422) antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞核,细胞浆,细胞膜
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated Synthesised phosphopeptide derived from human SGK1 around the phosphorylation site of Ser422
抗原表位：GF(p-S)YA
SWISS：O00141
Gene ID ：6446
Human Gene ID：6446
SGK1 is a protein kinase that plays an important role in cellular stress response. SGK1 activates certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion. Sustained high levels of SGK1 and activity may contribute to conditions such as hypertension and diabetic nephropathy. This protein also mediates cell survival signals, as it has been shown to phosphorylate and negatively regulate the pro apoptotic FOXO3A protein. Ser 422 is a critical site on the protein and may be involved in its activation.
Function：Protein kinase that plays an important role in cellular stress response. Activates certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability and renal sodium exc
Subunit：Interacts with NEDD4 and NEDD4L.
Subcellular Location：Isoform 2: Cell membrane.
Cytoplasm. Nucleus. Endoplasmic reticulum. Note=Nuclear, upon phosphorylation.
Tissue Specificity：Expressed in most tissues with highest levels in the pancreas, followed by placenta, kidney and lung. Isoform 2 is strongly expressed in brain and pancreas, weaker in heart, placenta, lung, liver and skeletal muscle.
Post-translational modifications：Regulated by phosphorylation. Phosphoinositide 3-kinase (PI3-kinase) pathway promotes phosphorylation at Ser-422 which in turn increases the phosphorylation of Thr-256 by PDPK1.
Ubiquitinated by NEDD4L; which promotes proteasomal degradation. Ubiquitin
Similarity：Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 protein kinase domain.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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