Rabbit Anti-Phospho-Lyn (Tyr397) antibody |
反应物种(预测) |
Chicken,Pig,Rabbit |
产品应用(已验证) |
WB,IHC |
产品应用(可尝试) |
IF,ELISA |
推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500, |
研究领域 |
免疫学,信号转导,激酶和磷酸酶 |
标签 |
Array |
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Paraformaldehyde-fixed, paraffin embedded (mouse spleen tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-Lyn (Tyr397)) Polyclonal Antibody, Unconjugated (bs-3257R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (rat embryo); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-Lyn (Tyr397)) Polyclonal Antibody, Unconjugated (bs-3257R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (rat spleen tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-Lyn (Tyr397)) Polyclonal Antibody, Unconjugated (bs-3257R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Sample:
Placenta (Mouse) Lysate at 40 ug
Lung (Mouse) Lysate at 40 ug
Primary: Anti-Phospho-Lyn (Tyr397)(bs-3257R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56kD
Observed band size: 63kD
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Paraformaldehyde-fixed, paraffin embedded (mouse lung tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-Lyn (Tyr397)) Polyclonal Antibody, Unconjugated (bs-3257R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
RRID:AB_10857141
产品名称:Rabbit Anti-Phospho-Lyn (Tyr397) antibody
别名: ALyn (phospho Y397); Lyn (phospho Tyr397); p-Lyn (Tyr397); Hck 2; JTK 8; JTK8; ONCOGENE LYN; Tyrosine protein kinase LYN; V yes 1 Yamaguchi sarcoma viral related oncogene homolog; Yamaguchi sarcoma viral (v yes 1) related oncogene homolog; AA407514; EC 2.
中文名称:磷酸化膜相关蛋白酪氨酸激酶Lyn抗体
英文名称:Rabbit Anti-Phospho-Lyn (Tyr397) antibody
抗体来源: Rabbit
克隆类型:多克隆
细胞定位:细胞核,细胞浆,细胞膜
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原:KLH conjugated synthesised phosphopeptide derived from human Lyn around the phosphorylation site of Tyr397
抗原表位:NE(p-Y)TA
SWISS:P07948
Gene ID :4067
Human Gene ID:4067
Lyn (also known as p53/56 Lyn) is a membrane associated protein tyrosine kinase (PTK) mostly expressed in hemopoietic cells which is important in cellular signaling. It contains an SH2 and SH3 domain and has been found to be cleaved after activation of caspases in apoptosis. A member of the Src family of PTKs, there are two known isoforms for Lyn which plays an indispensable role in the Fc epsilon RI (Fcer1) and the B cell IgM receptor signaling pathway and is essential for Syk activation and Lat phosphorylation after Fcer1 aggregation and can also phosphorylate Tec on multiple residues. Lyn can also be regulated by IL2 and IL3.
Function:Non-receptor tyrosine-protein kinase that transmits signals from cell surface receptors and plays an important role in the regulation of innate and adaptive immune responses, hematopoiesis, responses to growth factors and cytokines, integrin signaling, bu
Subunit:Interacts with TEC. Interacts (via SH2 domain) with FLT3 (tyrosine phosphorylated). Interacts with LIME1 and with CD79A upon activation of the B-cell antigen receptor. Interacts with the B-cell receptor complex. Interacts with phosphorylated THEMIS2. Inte
Subcellular Location:Cell membrane. Nucleus. Cytoplasm. Cytoplasm, perinuclear region. Golgi apparatus. Note=Accumulates in the nucleus by inhibition of CRM1-mediated nuclear export. Nuclear accumulation is increased by inhibition of its kinase activity. The trafficking from
Tissue Specificity:Detected in monocytes (at protein level). Detected in placenta, and in fetal brain, lung, liver and kidney. Widely expressed in a variety of organs, tissues, and cell types such as epidermoid, hematopoietic, and neuronal cells. Expressed in primary neurob
Post-translational modifications:Ubiquitinated by CBL, leading to its degradation. Ubiquitination is SH3-dependent.
Autophosphorylated. Phosphorylated on tyrosine residues in response to KIT signaling. Phosphorylation at Tyr-397 is required for optimal activity. Phosphorylation at Ty
DISEASE:Note=Constitutively phosphorylated and activated in cells from a number of chronic myelogenous leukemia (CML) and acute myeloid leukemia (AML) patients. Mediates phosphorylation of the BCR-ABL fusion protein. Abnormally elevated expression levels or activ
Similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.