Rabbit Anti-Phospho-CD19 (Tyr531) antibody |
反应物种(预测) |
Rat,Dog,Pig,Cow,Rabbit,GuineaPig |
产品应用(已验证) |
WB,FCM |
产品应用(可尝试) |
IHC,IF,ELISA |
推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=1μg /test,IF=1:100-500, |
研究领域 |
肿瘤,细胞生物,免疫学,信号转导,细胞表面分子,B-淋巴细胞, |
标签 |
Array |
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phosphopeptide
non phosphopeptide
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Blank control (blue line): HL60 cells (fixed with 70% methanol (Overnight at 4℃) . Cells stained with Primary Antibody for 30 min at room temperature).
Primary Antibody (green line): Rabbit Anti-CD19 antibody (bs-3065R),Dilution: 1μg /10^6 cells.
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE,Dilution: 1μg /test.
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Sample:
Lane 1: Human Raji cell lysates
Lane 2: Human K562 cell lysates
Primary: Anti-Phospho-CD19 (Tyr531) (bs-3065R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 59 kD
Observed band size: 105 kD
RRID:AB_10856818
产品名称:Rabbit Anti-Phospho-CD19 (Tyr531) antibody
别名: CD19_HUMAN; B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; Differentiation antigen CD19; T-cell surface antigen Leu-12; CD_antigen: CD19; B-lymphocyte surface antigen B4; Leu 12; Leu12;
中文名称:磷酸化CD19抗体
英文名称:Rabbit Anti-Phospho-CD19 (Tyr531) antibody
抗体来源: Rabbit
克隆类型:多克隆
细胞定位:细胞膜
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原:KLH conjugated Synthesised phosphopeptide derived from human CD19 around the phosphorylation site of Tyr531
抗原表位:DS(p-Y)EN
抗原细胞定位:Cytoplasmic
SWISS:P15391
Gene ID :930
Human Gene ID:930
CD19 is a transmembrane glycoprotein that is delectively expressed on the cell surface of B-lymphocytes,where it activates intracellular signaling cascades involving both Ras and phosphatidylinositol 3-kinase pathways.Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. This gene encodes a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.
Function:Assembles with the antigen receptor of B-lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.
Subunit:Forms a complex with CD21, CD81 and CD225 in the membrane of mature B-cells. Interacts with VAV. Interacts with GRB2 and SOS when phosphorylated on Tyr-348 and/or Tyr-378. Interacts with PLCG2 when phosphorylated on Tyr-409. Interacts with LYN.
Subcellular Location:Membrane; Single-pass type I membrane protein.
Post-translational modifications:Phosphorylated on serine and threonine upon DNA damage, probably by ATM or ATR. Phosphorylated on tyrosine following B-cell activation. Phosphorylated on tyrosine residues by LYN.
DISEASE:Defects in CD19 are the cause of immunodeficiency common variable type 3 (CVID3) [MIM:613493]; also called antibody deficiency due to CD19 defect. CVID3 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent b
Similarity:Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.