| Rabbit Anti-ABCB5 antibody |
| 反应物种（预测） |
Rat,Dog,Cow,Horse |
| 产品应用（已验证） |
WB,IF |
| 产品应用（可尝试） |
IHC,ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500, |
| 研究领域 |
肿瘤,信号转导,通道蛋白,糖蛋白, |
| 标签 |
Array |
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Sample:
Lane 1: Mouse B16 cell lysates
Lane 2: Mouse Testis tissue lysates
Primary: Anti-ABCB5 (bs-1604R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 89/138 kD
Observed band size: 125 kD
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Tissue/cell: mouse melanoma tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ABCB5 Polyclonal Antibody, Unconjugated(bs-1604R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
RRID：AB_10856247
产品名称：Rabbit Anti-ABCB5 antibody
别名： ATP-binding cassette sub-family B member 5; ABCB 5; ABCB5alpha; ABCB5beta; ATP binding cassette sub family B (MDR/TAP) member 5; ATP binding cassette sub family B member 5; EST422562; P glycoprotein ABCB5; ABCB5_HUMAN.
中文名称：ATP结合蛋白家族5抗体
英文名称：Rabbit Anti-ABCB5 antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞膜
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human ABCB5
抗原表位：841-883/1257
抗原细胞定位：Cytoplasmic
SWISS：Q2M3G0
Gene ID ：340273
Human Gene ID：340273
ABCB5 belongs to the ATP-binding cassette (ABC) transporter superfamily of integral membrane proteins. These proteins participate in ATP-dependent transmembrane transport of structurally diverse molecules ranging from small ions, sugars, and peptides to more complex organic molecules (Chen et al., 2005 [PubMed 15760339]).[supplied by OMIM, Mar 2008]
Function：Plasma membrane transporter able to mediate efflux from cells of the rhodamine dye and of the therapeutic drug doxorubicin. Responsible for the resistance to doxorubicin of a subset of malignant melanomas.
Subcellular Location：Cell membrane; Multi-pass membrane protein.
Tissue Specificity：Expressed by CD133-expressing progenitor cells among epidermal melanocytes (at protein level). Widely expressed with specific expression in pigment cells. Also expressed in several malignant tissues.
Similarity：Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.
Contains 1 ABC transmembrane type-1 domain.
Contains 2 ABC transporter domains.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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