| Rabbit Anti-5HT2A Receptor antibody |
| 反应物种（预测） |
Dog,Pig,Cow,Horse,Sheep |
| 产品应用（已验证） |
WB,IHC,ELISA |
| 产品应用（可尝试） |
ICC,IF |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500, |
| 研究领域 |
心血管,细胞生物,神经生物学,细胞膜受体,新陈代谢,G蛋白偶联受体,G蛋白信号, |
| 标签 |
Array |
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Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-5HT2A Polyclonal Antibody, Unconjugated(bs-12049R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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Sample:Cerebellum (Mouse) Lysate at 40 ug
Primary: Anti-5HT2A Receptor (bs-12049R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 53 kD
Observed band size: 53 kD
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Sample: Small intestine (Mouse) Lysate at 40 ug
Primary: Anti-5HT2A Receptor (bs-12049R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 53 kD
Observed band size: 53 kD
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Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (5HT2A Receptor) Polyclonal Antibody, Unconjugated (bs-12049R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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Sample:
Lane 1: Cerebrum (Mouse) Lysate at 40 ug
Lane 2: Cerebrum (Rat) Lysate at 40 ug
Primary: Anti-5HT2A Receptor (bs-12049R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 53 kD
Observed band size: 50 kD
RRID：RRID
产品名称：Rabbit Anti-5HT2A Receptor antibody
别名： SR-2A; 5 HT 2; 5 HT 2A; 5 HT2 receptor; 5 HT2A; 5 hydroxytryptamine receptor 2A; 5-HT-2; 5-HT-2A; 5-hydroxytryptamine 2A receptor; 5-hydroxytryptamine receptor 2A; 5HT2A_HUMAN; HTR 2; HTR 2A; HTR2; HTR2A; Serotonin receptor 2A.
中文名称：5-羟色胺受体2A抗体
英文名称：Rabbit Anti-5HT2A Receptor antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞膜
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human 5HT2A Receptor
抗原表位：51-150/471
抗原细胞定位：Extracellular
SWISS：P28223
Gene ID ：3356
Human Gene ID：3356
This gene encodes one of the receptors for serotonin, a neurotransmitter with many roles. Mutations in this gene are associated with susceptibility to schizophrenia and obsessive-compulsive disorder, and are also associated with response to the antidepressant citalopram in patients with major depressive disorder (MDD). MDD patients who also have a mutation in intron 2 of this gene show a significantly reduced response to citalopram as this antidepressant downregulates expression of this gene. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]
Function：This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor mediates its action by association with G proteins that activate a phospha
Subunit：Interacts with MPDZ and INADL. May interact with MPP3, PRDX6, DLG4, DLG1, CASK, APBA1 and MAGI2.
Subcellular Location：Cell membrane. Localizes to the post-synaptic thickening of axo-dendritic synapses.
Similarity：Belongs to the G-protein coupled receptor 1 family.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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