Rabbit Anti-Phospho-FGFR1 (Tyr653 + Tyr654) antibody |
反应物种(预测) |
Dog,Pig,Cow,Horse,Rabbit |
产品应用(已验证) |
WB,IHC |
产品应用(可尝试) |
ELISA |
推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500, |
研究领域 |
肿瘤,免疫学,生长因子和激素,转录调节因子 |
标签 |
Array |
-
Paraformaldehyde-fixed, paraffin embedded (human laryngeal carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-FGFR1 (Tyr653 + Tyr654)) Polyclonal Antibody, Unconjugated (bs-3205R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
-
Tissue/cell: rat liver tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Phospho-FGFR1 (Tyr653 + Tyr654) Polyclonal Antibody, Unconjugated(bs-3205R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
-
Sample:
MCF-7(Human) Cell Lysate at 30 ug
K562(Human) Cell Lysate at 30 ug
Primary: Anti- Phospho-FGFR1(Tyr653 + Tyr654) (bs-3205R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 88 kD
Observed band size: 128 kD
-
Sample:
Embryo (Mouse) Lysate at 40 ug
Primary: Anti- Phospho-FGFR1(Tyr653 + Tyr654) (bs-3205R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 88 kD
Observed band size: 128 kD
RRID:AB_10857184
产品名称:Rabbit Anti-Phospho-FGFR1 (Tyr653 + Tyr654) antibody
别名: FGFR1 alpha (phospho Y654) ; FGFR1 (phospho Y653 + Y654); p-FGFR1 (phospho Y653 + Y654); bFGF R; BFGFR; C FGR; CD 331; CD331; CD331 antigen; FGF Receptor; CEK; FGFBR; FGFR 1; Fibroblast growth factor receptor 1; FLG; FLG protein; FLJ14326; FLT 2; FLT2; Fm
中文名称:磷酸化碱性成纤维细胞生长因子受体1(CD331)抗体
英文名称:Rabbit Anti-Phospho-FGFR1 (Tyr653 + Tyr654) antibody
抗体来源: Rabbit
克隆类型:多克隆
细胞定位:细胞核,细胞浆,细胞膜
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原:KLH conjugated Synthesised phosphopeptide derived from human FGFR1 around the phosphorylation site of Tyr653/654
抗原表位:ID(p-Y)(p-Y)KK
抗原细胞定位:Cytoplasmic
SWISS:P11362
Gene ID :2260
Human Gene ID:2260
Fibroblast growth factors (FGFs) produce mitogenic and angiogenic effects in target cells by signaling through the cellular surface tyrosine kinase receptors. There are four members of the FGF receptor family: FGFR-1 (flg), FGFR-2 (bek, KGFR), FGFR-3 and FGFR-4. Each receptor contains an extracellular ligand binding domain, a transmembrane region and a cytoplasmic kinase domain (1). Following ligand binding and dimerization, the receptors are phosphorylated at specific tyrosine residues (2). Seven tyrosine residues in the cytoplasmic tail of FGFR-1 can be phosphorylated: Tyr463, Tyr583, Tyr585, Tyr653, Tyr654, Tyr730 and Tyr766. Tyrosine 653 and 654 are important for catalytic activity of the activated FGFR and are essential for signaling (3). The other phosphorylated tyrosine residues may provide docking sites for downstream signaling components such as Crk and PLCgamma.
Function:Receptor for fibroblast growth factors FGF2 and FGF1. Receptor for FGF23 in the presence of KL. Promotes mitogenesis in response to fibroblast growth factors. Activates PLCG1.
Subunit:Homodimer. Interacts with KLB. Interacts with KL and FGF23. Interacts with SHB and GRB10. Interacts with PLCG1 (via SH2 domains). Interacts with KAL1; this interaction does not interfere with FGF2-binding to FGFR1, but prevents binding of heparin-bound FG
Subcellular Location:Cell membrane; Single-pass type I membrane protein. Nucleus. Cytoplasm, cytosol. Cytoplasmic vesicle.
Tissue Specificity:Detected in astrocytoma, neuroblastoma and adrenal cortex cell lines. Some isoforms are detected in foreskin fibroblast cell lines, however isoform 17, isoform 18 and isoform 19 are not detected in these cells.
Post-translational modifications:Binding of FGF1 and heparin promotes autophosphorylation on tyrosine residues and activation of the receptor.
DISEASE:Defects in FGFR1 are a cause of Pfeiffer syndrome (PS) [MIM:101600]; also known as acrocephalosyndactyly type V (ACS5). PS is characterized by craniosynostosis (premature fusion of the skull sutures) with deviation and enlargement of the thumbs and great
Similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family. Fibroblast growth factor receptor subfamily.
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.