| Rabbit Anti-phospho-PPAR alpha (Ser12) antibody |
| 反应物种（预测） |
Dog,Pig,Cow,Horse,GuineaPig |
| 产品应用（已验证） |
WB,IHC |
| 产品应用（可尝试） |
IF,ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500, |
| 研究领域 |
肿瘤,细胞生物,免疫学,转录调节因子,激酶和磷酸酶 |
| 标签 |
Array |
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Sample:
Colon carcinoma (Human) Lysate at 40 ug
Embryo (Mouse) Lysate at 40 ug
Primary: Anti- phospho-PPAR alpha (Ser12) (bs-4055R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
Predicted band size: 52 kD
Observed band size: 52 kD
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Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-phospho-PPAR alpha(Ser12) Polyclonal Antibody, Unconjugated(bs-4055R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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phosphopeptide
non phosphopeptide
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Sample:
Liver (Rat) Lysate at 40 ug
Primary: Anti-phospho-PPAR alpha (Ser12) (bs-4055R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kD
Observed band size: 52 kD
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Sample:
Lane 1: Heart (Mouse) Lysate at 40 ug
Lane 2: Liver (Mouse) Lysate at 40 ug
Lane 3: Kidney (Mouse) Lysate at 40 ug
Lane 4: Cerebrum (Mouse) Lysate at 40 ug
Lane 5: Cerebrum (Rat) Lysate at 40 ug
Lane 6: Jurkat (Human) Cell Lysate at 30 ug
Lane 7: A431 (Human) Cell Lysate at 30 ug
Primary: Anti-phospho-PPAR alpha (Ser12) (bs-4055R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 53 kD
Observed band size: 50 kD
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Sample:
Heart (Mouse) Lysate at 40 ug
Liver (Mouse) Lysate at 40 ug
Primary: Anti-phospho-PPAR alpha (Ser12) (bs-4055R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kD
Observed band size: 52 kD
RRID：AB_10855872
产品名称：Rabbit Anti-phospho-PPAR alpha (Ser12) antibody
别名： PPAR alpha (phospho-Ser12); PPAR alpha (phospho-S12); p-PPAR alpha (Ser12); p-PPAR alpha (S12); hPPAR; MGC2237; MGC2452; NR1C1; Nuclear receptor subfamily 1 group C member 1; Peroxisome Proliferator Activated Receptor alpha; PPAR; PPARA; Peroxisome prolif
中文名称：磷酸化α型-过氧化酶活化增生受体抗体
英文名称：Rabbit Anti-phospho-PPAR alpha (Ser12) antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞核
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthesised phosphopeptide derived from human PPAR alpha around the phosphorylation site of ser12
抗原表位：PL(p-S)PL
SWISS：Q07869
Gene ID ：5465
Human Gene ID：5465
Peroxisome proliferators are nongenotoxic carcinogens which are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family, termed Peroxisome Proliferator Activated Receptors (PPARs). Nuclear hormone receptors are ligand dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Studies indicate that PPARs are activated by peroxisome proliferators such as clofibric acid, nafenopin, and WY-14,643, as well as by some fatty acids. It has also been shown that PPARs can induce transcription of acyl coenzyme A oxidase and cytochrome P450 A6 (CYP450 A6) through interaction with specific response elements. PPAR alpha is activated by free fatty acids including linoleic, arachidonic, and oleic acids. Induction of peroxisomes by this mechanism leads to a reduction in blood triglyceride levels. PPAR alpha is expressed mainly in skeletal muscle, heart, liver, and kidney and is thought to regulate many genes involved in the beta-oxidation of fatty acids. Activation of rat liver PPAR alpha has been shown to suppress hepatocyte apoptosis. PPAR alpha, like several other nuclear hormone receptors, heterodimerizes with retinoic X receptor (RXR) alpha to form a transcriptionally competent complex.
Function：Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates sati
Subunit：Heterodimer; with RXRA. This heterodimerization is required for DNA binding and transactivation activity. Interacts with AKAP13, LPIN1 and PRDM16. Also interacts with PPARBP coactivator in vitro. Interacts with CITED2; the interaction stimulates its trans
Subcellular Location：Nucleus.
Tissue Specificity：Skeletal muscle, liver, heart and kidney.
Similarity：Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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