| Rabbit Anti-phospho-MEK1 (Thr386) antibody |
| 反应物种（预测） |
Chicken,Pig,Horse |
| 产品应用（已验证） |
WB,IHC |
| 产品应用（可尝试） |
IF,ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500, |
| 研究领域 |
肿瘤,免疫学,信号转导,转录调节因子,激酶和磷酸酶 |
| 标签 |
Array |
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Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MEK1 (Thr386)) Polyclonal Antibody, Unconjugated (bs-5413R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MEK1 (Thr386)) Polyclonal Antibody, Unconjugated (bs-5413R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Sample:
placenta (Mouse) Lysate at 40 ug
Primary: Anti-phospho-MEK1 (Thr386) (Bs- 5413R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43 kD
Observed band size: 48 kD
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Sample:
Lane 1: Mouse Brain Lysates
Lane 2: Mouse Adrenal gland Lysates
Lane 3: Rat Brain Lysates
Lane 4: Rat Adrenal gland Lysates
Lane 5: Human DU145 cell Lysates
Lane 6: Human HepG2 cell Lysates
Lane 7: Human A431 cell Lysates
Lane 8: Human HeLa cell Lysates
Primary: Anti-phospho-MEK1 (Thr386) (bs-5413R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43kDa
Observed band size: 50kDa
RRID：AB_11075937
产品名称：Rabbit Anti-phospho-MEK1 (Thr386) antibody
别名： MEK1 (phospho Thr386); MEK1 (phospho T386); p-MEK1 (Thr386); p-MEK1 (T386); Dual specificity mitogen activated protein kinase kinase 1; ERK activator kinase 1; MAP kinase kinase 1; MAP kinase/Erk kinase 1; MAP/Erk kinase 1; Map2K1; MAPK/ERK kinase 1; MAPK
中文名称：磷酸化丝裂原活化蛋白激酶1抗体
英文名称：Rabbit Anti-phospho-MEK1 (Thr386) antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞核,细胞浆,细胞膜
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated Synthesised phosphopeptide derived from human MEK1 around the phosphorylation site of Thr386
抗原表位：SP(p-T)PT
SWISS：Q02750
Gene ID ：5604
Human Gene ID：5604
The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development.
Function：Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this init
Subunit：Found in a complex with at least BRAF, HRAS1, MAP2K1, MAPK3/ERK1 and RGS14 (By similarity). Forms an heterodimer with MAP2K2/MEK2 (By similarity). Forms heterodimers with KSR2 which further dimerize to form tetramers (By similarity). Interacts with ARBB2,
Subcellular Location：Cytoplasm, cytoskeleton, centrosome. Cytoplasm, cytoskeleton, spindle pole body. Cytoplasm. Nucleus. Note=Localizes at centrosomes during prometaphase, midzone during anaphase and midbody during telophase/cytokinesis.
Tissue Specificity：Widely expressed, with extremely low levels in brain.
Post-translational modifications：Phosphorylation at Ser-218 and Ser-222 by MAP kinase kinase kinases (RAF or MEKK1) positively regulates kinase activity. Also phosphorylated at Thr-292 by MAPK1/ERK2 and at Ser-298 by PAK. MAPK1/ERK2 phosphorylation of Thr-292 occurs in response to cellul
Similarity：Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
Contains 1 protein kinase domain.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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