Rabbit Anti-MBOAT4 antibody |
反应物种(预测) |
Human,Dog,Horse,Rabbit,Monkey,Gorilla |
产品应用(已验证) |
WB,IHC |
产品应用(可尝试) |
ICC,IF,ELISA |
推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500, |
研究领域 |
细胞生物 |
标签 |
Array |
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Sample: stomach (mouse) Lysate at 40 ug
Primary: Anti- MBOAT4(bs-13355R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50kD
Observed band size: 55 kD
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Paraformaldehyde-fixed, paraffin embedded (Rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MBOAT4) Polyclonal Antibody, Unconjugated (bs-13355R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
RRID:RRID
产品名称:Rabbit Anti-MBOAT4 antibody
别名: FKSG89; ghrelin o transferas; Ghrelin O-acyltransferase; GOAT; MBOA4_HUMAN; Mboat4; Membrane-bound O-acyltransferase domain-containing protein 4; O-acyltransferase (membrane bound) domain containing 4; O-acyltransferase domain-containing protein 4; OACT4.
中文名称:脑肠肽O酰基转移酶/Ghrelin O acyltransferase抗体
英文名称:Rabbit Anti-MBOAT4 antibody
抗体来源: Rabbit
克隆类型:多克隆
细胞定位:细胞浆,细胞膜
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原:KLH conjugated synthetic peptide derived from human Ghrelin O acyltransferase
抗原表位:101-200/435
SWISS:Q96T53
Gene ID :619373
Human Gene ID:619373
MBOAT4 (membrane-bound O-acyltransferase domain-containing protein 4, Ghrelin O-acyltransferase) is a 435 amino acid, multi-pass membrane protein that belongs to the membrane-bound acyltransferase family. MBOAT4 functions as an enzyme that attaches an octanoate fatty acid to serine-3 of ghrelin. Ghrelin is a very small, appetite-stimulating hormone secreted by the food-deprived stomach. MBOAT4 can use a variety of fatty acids as substrates including octanoic acid, decanoic acid and tetradecanoic acid. MBOAT4 expression, consistent with its function, is mainly in the stomach and intestines. Due to its primary function, MBOAT4 is a candidate for obesity and appetite suppression studies.
Function:Mediates the octanoylation of ghrelin at 'Ser-3'. Can use a variety of fatty acids as substrates including octanoic acid, decanoic acid and tetradecanoic acid.
Subcellular Location:Endoplasmic reticulum membrane; Multi-pass membrane protein.
Tissue Specificity:Expressed predominantly in stomach with moderate levels in pancreas and relatively low levels in most other tissues.
Similarity:Belongs to the membrane-bound acyltransferase family.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.