| Rabbit Anti-phospho-p107 (Ser975) antibody |
| 反应物种（预测） |
Dog,Pig,Cow,Horse,Sheep |
| 产品应用（已验证） |
WB,IHC |
| 产品应用（可尝试） |
IF,ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500, |
| 研究领域 |
肿瘤,免疫学,信号转导,干细胞,细胞周期蛋白,转录调节因子 |
| 标签 |
Array |
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Sample:
Testis (Rat) Lysate at 40 ug
Primary: Anti- phospho-p107(Ser975) (bs-5696R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 121 kD
Observed band size: 121 kD
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Paraformaldehyde-fixed, paraffin embedded (rat lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-p107 (Ser975)) Polyclonal Antibody, Unconjugated (bs-5696R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Sample: K562 Cell (Human) Lysate at 40 ug
Primary: Anti-phospho-p107(Ser975) (bs-5696R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 121 kDa
Observed band size: 121 kDa
RRID：AB_11097514
产品名称：Rabbit Anti-phospho-p107 (Ser975) antibody
别名： RBL1(phospho Ser975); RBL1(phospho S975); Cellular protein 107; CP107; PRB1; RBL1; Retinoblastoma like protein 1; RBL1_HUMAN.
中文名称：磷酸化视网膜母细胞瘤样蛋白p107抗体
英文名称：Rabbit Anti-phospho-p107 (Ser975) antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞核
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated Synthesised phosphopeptide derived from human RBL1 around the phosphorylation site of Ser975
抗原表位：PG(P-S)PR
SWISS：P28749
Gene ID ：5933
Human Gene ID：5933
The pocket protein family consists of three structurally and functionally related proteins, Rb (retinoblastoma), p107, and p130. This family of tumor suppressors function to regulate important cellular transcription factors, such as the E2F family. The E2F proteins regulate the expression of genes whose products are important for cell cycle progression. The inactivation Rb is catalyzed by CDK phosphorylation thereby releasing E2F during the G1-S phase cellular progression. Unchecked inactivation of Rb in G1 phase has been indicated as a universal mechanism underlying cellular transformation . While Rb has been the most studied among the pocket proteins, p107 and p130 have also been shown to be key regulators of E2F. Several studies have also provided evidence that p107/p130 provide different functions in E2F regulation than does Rb. Rb, p107, and p130 each contain a conserved 'A/B pocket', which is the target of several viral oncoproteins, namely SV40 large T-antigen and adenovirus E1A. There are two isoforms.
Function：Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone
Subunit：Interacts with SUV420H1, SUV420H2 and USP4 (By similarity). Component of the DREAM complex (also named LINC complex) at least composed of E2F4, E2F5, LIN9, LIN37, LIN52, LIN54, MYBL1, MYBL2, RBL1, RBL2, RBBP4, TFDP1 and TFDP2. The complex exists in quiesc
Subcellular Location：Nucleus.
Post-translational modifications：Exists in both phosphorylated and unphosphorylated forms, and T, but not E1A, binds only to the unphosphorylated form. Cell-cycle arrest properties are inactivated by phosphorylation on Thr-332, Ser-640, Ser-964 and Ser-975 by CDK4.
Similarity：Belongs to the retinoblastoma protein (RB) family.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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