Rabbit Anti-AMACR antibody |
产品应用(已验证) |
WB,IHC |
产品应用(可尝试) |
IF,ELISA |
推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500, |
研究领域 |
肿瘤,细胞生物,免疫学,染色质和核信号,信号转导,新陈代谢,表观遗传学, |
标签 |
Array |
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Sample:
Lane 1: Mouse Liver tissue lysates
Lane 2: Mouse Kidney tissue lysates
Lane 3: Rat Liver tissue lysates
Lane 4: Rat Kidney tissue lysates
Primary: Anti-AMACR (bs-0840R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kDa
Observed band size: 38 kDa
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Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (AMACR) Polyclonal Antibody, Unconjugated (bs-0840R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
RRID:AB_10885747
产品名称:Rabbit Anti-AMACR antibody
别名: Alpha-methylacyl-CoA racemase; 2-methylacyl-CoA racemase; RM; RACE; CBAS4; P504S; AMACRD; AMACR_HUMAN;
中文名称:α-甲基酰基辅酶A消旋酶抗体
英文名称:Rabbit Anti-AMACR antibody
抗体来源: Rabbit
克隆类型:多克隆
细胞定位:细胞浆
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原:KLH conjugated synthetic peptide derived from mouse AMACR
抗原表位:1-100/381
SWISS:Q9UHK6
Gene ID :23600
Human Gene ID:23600
This gene encodes a racemase. The encoded enzyme interconverts pristanoyl-CoA and C27-bile acylCoAs between their (R)- and (S)-stereoisomers. The conversion to the (S)-stereoisomers is necessary for degradation of these substrates by peroxisomal beta-oxidation. Encoded proteins from this locus localize to both mitochondria and peroxisomes. Mutations in this gene may be associated with adult-onset sensorimotor neuropathy, pigmentary retinopathy, and adrenomyeloneuropathy due to defects in bile acid synthesis. Alternatively spliced transcript variants have been described. Read-through transcription also exists between this gene and the upstream neighboring C1QTNF3 (C1q and tumor necrosis factor related protein 3) gene. [provided by RefSeq, Mar 2011]
Function:Racemization of 2-methyl-branched fatty acid CoA esters. Responsible for the conversion of pristanoyl-CoA and C27-bile acyl-CoAs to their (S)-stereoisomers.
Subcellular Location:Peroxisome. Mitochondrion.
DISEASE:Alpha-methylacyl-CoA racemase deficiency (AMACRD) [MIM:614307]: A rare autosomal recessive peroxisomal disorder characterized by elevated plasma concentrations of pristanic acid C27-bile-acid intermediates, and adult onset of variable neurodegenerative sy
Similarity:Belongs to the CaiB/BaiF CoA-transferase family.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.