| Rabbit Anti-CREB3L3 antibody |
| 反应物种（预测） |
Mouse,Rat,Chicken,Dog,Pig,Cow,Sheep |
| 产品应用（已验证） |
FCM |
| 产品应用（可尝试） |
WB,IHC,ICC,IF,ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=0.2ug/Test,IF=1:100-500,ICC=1:100-500, |
| 研究领域 |
转录调节因子,表观遗传学, |
| 标签 |
Array |
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Blank control: HepG2.
Primary Antibody (green line): Rabbit Anti-CREB3L3 antibody (bs-11239R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
RRID：RRID
产品名称：Rabbit Anti-CREB3L3 antibody
别名： cAMP responsive element binding protein 3 like 3; cAMP responsive element-binding protein 3 like protein 3; CREB H; CREB/ATF family transcription factor; CREBH; Cyclic AMP responsive element binding protein 3 like protein 3; cAMP-responsive element-bindin
中文名称：环腺苷酸应答元件结合蛋白H抗体
英文名称：Rabbit Anti-CREB3L3 antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞核,细胞浆,细胞膜
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human CREB-H
抗原表位：201-300/461
SWISS：Q68CJ9
Gene ID ：84699
Human Gene ID：84699
CREB3L3 is a 461 amino acid single-pass type II membrane protein that localizes to the endoplasmic reticulum (ER) and, in response to ER stress, is cleaved and translocated to the nucleus. Expressed exclusively in liver, CREB3L3 functions as a transcription factor that, during ER stress, is thought to activate genes that are involved in both the unfolded protein response and the acute phase response (APR). Additionally, CREB3L3 is underexpressed in hepatocellular carcinoma, suggesting a possible role as a tumor suppressor. CREB3L3 functions as a dimer and contains one leucine zipper domain, a KDEL-like sequence and a bZIP domain, through which it conveys its DNA binding ability. Three isoforms of CREB3L3 exist due to alternative splicing events.
Function：Transcription factor that may act during endoplasmic reticulum stress by activating unfolded protein response target genes. Activated in response to cAMP stimulation. In vitro, binds to the cAMP response element (CRE) and box-B element. Activates transcri
Subunit：Binds DNA as a dimer (By similarity). Probably homodimerizes. Probably forms a heterodimer with ATF6. Interacts with ATF6.
Subcellular Location：Endoplasmic reticulum membrane; Single-pass type II membrane protein. Nucleus.
Tissue Specificity：Exclusively expressed in liver. Underexpressed in hepatocellular carcinoma tissues.
Post-translational modifications：Controlled by regulated intramembrane proteolysis (RIP). Following ER stress a fragment containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage seems to be performed sequentially by site-1 and site-2 proteases (PS1
Similarity：Belongs to the bZIP family. ATF subfamily.
Contains 1 bZIP (basic-leucine zipper) domain.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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