Rabbit Anti-Phospho-PSD95 (Tyr236 + Tyr240) antibody |
反应物种(预测) |
Dog,Pig,Cow,Rabbit |
产品应用(已验证) |
IHC,FCM |
产品应用(可尝试) |
IF,ELISA |
推荐稀释比例 |
Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=1μg /Test,IF=1:100-500, |
研究领域 |
免疫学,神经生物学,细胞类型标志物(Synaptic Marker), |
标签 |
Array |
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Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-P70 S6 beta) Polyclonal Antibody, Unconjugated (bs-3349R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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Blank control (blue line): mouse brain (fixed with 2% paraformaldehyde for 10 min at room temperature, and then stained with Primary Antibody for 30 min at room temperature).
Primary Antibody (green line): Rabbit Anti-Phospho-PSD95 (Tyr236 + Tyr240)antibody (bs-3349R),Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FTIC,Dilution: 1μg /test.
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Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-PSD95 (Tyr236 + Tyr240)) Polyclonal Antibody, Unconjugated (bs-3349R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (Rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-PSD95 (Tyr236 + Tyr240)) Polyclonal Antibody, Unconjugated (bs-3349R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
RRID:AB_10858038
产品名称:Rabbit Anti-Phospho-PSD95 (Tyr236 + Tyr240) antibody
别名: PSD95 (Phospho Tyr236 + Tyr240); PSD95 (Phospho Y236 + Y240); Discs large homolog 4; Disks large homolog 4; DLG 4; DLG4; DLG4_HUMAN; FLJ97752; FLJ98574; Human post synaptic density protein 95; Human post-synaptic density protein 95; Post synaptic density
中文名称:磷酸化突触后密度蛋白95抗体
英文名称:Rabbit Anti-Phospho-PSD95 (Tyr236 + Tyr240) antibody
抗体来源: Rabbit
克隆类型:多克隆
细胞定位:细胞浆,细胞膜
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原:KLH conjugated Synthesised phosphopeptide derived from human PSD95 around the phosphorylation site of Tyr236/Tyr240
抗原表位:NT(p-Y)DV
SWISS:P78352
Gene ID :1742
Human Gene ID:1742
This gene encodes a member of the membrane-associated guanylate kinase (MAGUK) family. It heteromultimerizes with another MAGUK protein, DLG2, and is recruited into NMDA receptor and potassium channel clusters. These two MAGUK proteins may interact at postsynaptic sites to form a multimeric scaffold for the clustering of receptors, ion channels, and associated signaling proteins. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Function:Interacts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory s
Subunit:Interacts with ANKS1B, KLHL17 and PRR7. Interacts through its PDZ domains with NETO1. Interacts through its first two PDZ domains with GRIN2A, GRIN2B, GRIN2C, GRIN2D, ACCN3, certain splice forms of GRIN1, KCND2, CXADR and SYNGAP1. Interacts through its se
Subcellular Location:Cell membrane; Peripheral membrane protein. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell junction, synapse. Cell junction, synapse, synaptosome (By similarity).
Tissue Specificity:Brain.
Similarity:Belongs to the MAGUK family.
Contains 1 guanylate kinase-like domain.
Contains 3 PDZ (DHR) domains.
Contains 1 SH3 domain.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.