Rabbit Anti-DNase II antibody,蛋白Marker-蛋白检测试剂盒-IVD原料-北京博奥森生物技术有限公司

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Rabbit Anti-DNase II antibody

多克隆 | SKU：bs-7652R

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货号:bs-3351R

￥1280

50μg

RMB1280

100μg

RMB1980

200μg

RMB2980

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产品信息
免疫原信息
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订购号:bs-7652R

￥1138.00-2900.00

50ul

100ul

200ul

货期：现货

售后保障

Rabbit Anti-DNase II antibody
反应物种（预测）	Rat,Dog,Pig,Cow,Sheep
产品应用（已验证）	IHC,IF
产品应用（可尝试）	ELISA
推荐稀释比例	Elisa=1:5000-10000,IHC-P=1:100-500,IF=1:100-500,
研究领域	细胞生物,细胞凋亡,细胞周期蛋白,表观遗传学,
标签	Array

Paraformaldehyde-fixed, paraffin embedded (Mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DNase II) Polyclonal Antibody, Unconjugated (bs-7652R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: human colon cancer;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min;
Incubation: Anti-DNase II Polyclonal Antibody, Unconjugated(bs-7652R) 1:200, overnight at 4∑C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37∑C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei

产品信息

RRID：RRID
产品名称：Rabbit Anti-DNase II antibody
别名： Acid DNase; Deoxyribonuclease 2 alpha; Deoxyribonuclease 2; Deoxyribonuclease II alpha; Deoxyribonuclease II; Deoxyribonuclease II lysosomal; Deoxyribonuclease-2-alpha; Deoxyribonuclease2; DeoxyribonucleaseII; DNASE 2; DNASE 2A; DNase II alpha; DNase II l
中文名称：脱氧核糖核酸酶2抗体
英文名称：Rabbit Anti-DNase II antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞浆
性状：Liquid
亚型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

免疫原信息

免疫原：KLH conjugated synthetic peptide derived from human DNase II
抗原表位：25-90/360
SWISS：O00115
Gene ID ：1777
Human Gene ID：1777

产品介绍

Hydrolyzes DNA under acidic conditions with a preference for double-stranded DNA. Plays a major role in the degradation of nuclear DNA in cellular apoptosis during development. Necessary for proper fetal development and for definitive erythropoiesis in fetal liver, where it degrades nuclear DNA expelled from erythroid precursor cells.
Function：Hydrolyzes DNA under acidic conditions with a preference for double-stranded DNA. Plays a major role in the degradation of nuclear DNA in cellular apoptosis during development. Necessary for proper fetal development and for definitive erythropoiesis in fe
Subcellular Location：Lysosome.
Post-translational modifications：Glycosylated. Mutations that eliminate N-glycosylation sites reduce activity, but enzymatic deglycosylation has no effect.
Similarity：Belongs to the DNase II family.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.