Rabbit Anti-phospho MCSF Receptor (Tyr699) antibody |
反应物种(预测) |
Mouse,Rat,Dog |
产品应用(可尝试) |
ELISA |
推荐稀释比例 |
Elisa=1:5000-10000, |
研究领域 |
细胞生物,细胞膜受体 |
标签 |
Array |
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Cell: U937.
Concentration: 1:100.
Incubation: 40 minutes, room temperature.
Host/Blank: U937 cells.
Flow cytometric analysis of Rabbit Anti-phospho MCSF Receptor (Tyr699) antibody (bs-3075R) (green) compared with control in the absence of primary antibody (blue) followed by U937 cells.
secondary antibody: Goat Anti-rabbit IgG/FITC antibody (bs-0295G-FITC)
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Blank control: Raw264.7.
Primary Antibody (green line): Rabbit Anti-phospho MCSF Receptor (Tyr699) antibody (bs-3075R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
RRID:AB_10855206
产品名称:Rabbit Anti-phospho MCSF Receptor (Tyr699) antibody
别名: MCSF Receptor; C FMS; CD 115; CD115; CD115 antigen; CFMS; Colony stimulating factor 1 receptor; Colony stimulating factor I receptor; CSF 1 R; CSF 1R; CSF-1 receptor; CSF-1-R; CSF1 R; CSF1R; CSF1R_HUMAN; CSFR; FIM 2; FIM2; FMS; FMS proto oncogene; FMS pro
中文名称:磷酸化巨噬细胞集落刺激因子受体抗体
英文名称:Rabbit Anti-phospho MCSF Receptor (Tyr699) antibody
抗体来源: Rabbit
克隆类型:多克隆
细胞定位:细胞膜
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原:KLH conjugated Synthesised phosphopeptide derived from human CSF1R around the phosphorylation site of Tyr699
抗原表位:Ac-(p-Y)KN
SWISS:P07333
Gene ID :1436
Human Gene ID:1436
This protein tyrosine kinase transmembrane receptor is the receptor for colony stimulating factor 1, a cytokine which controls the production, differentiation, and function of macrophages. This receptor mediates most if not all of the biological effects of this cytokine. Ligand binding activates the receptor kinase through a process of oligomerization and transphosphorylation. The encoded protein is a member of the CSF1/PDGF receptor family of tyrosine protein kinases and contains 5 immunoglobulin like C2 type domains. CD115 is expressed by cells of the monocytic lineage and by progenitor cells. Mutations in this gene have been associated with a predisposition to myeloid malignancy.
Function:Tyrosine-protein kinase that acts as cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macropha
Subunit:Interacts with INPPL1/SHIP2 and THOC5 (By similarity). Monomer. Homodimer. Interacts with CSF1 and IL34. Interaction with dimeric CSF1 or IL34 leads to receptor homodimerization. Interacts (tyrosine phosphorylated) with PLCG2 (via SH2 domain). Interacts (
Subcellular Location:Cell membrane; Single-pass type I membrane protein.
Tissue Specificity:Expressed in bone marrow and in differentiated blood mononuclear cells.
Post-translational modifications:Autophosphorylated in response to CSF1 or IL34 binding. Phosphorylation at Tyr-561 is important for normal down-regulation of signaling by ubiquitination, internalization and degradation. Phosphorylation at Tyr-561 and Tyr-809 is important for interaction
DISEASE:Note=Aberrant expression of CSF1 or CSF1R can promote cancer cell proliferation, invasion and formation of metastases. Overexpression of CSF1 or CSF1R is observed in a significant percentage of breast, ovarian, prostate, and endometrial cancers.
Note=
Similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.