Rabbit Anti-phospho-MAPK8 ( Thr183 + Tyr185) antibody,蛋白Marker-蛋白检测试剂盒-IVD原料-北京博奥森生物技术有限公司

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磷酸化抗体

Rabbit Anti-phospho-MAPK8 ( Thr183 + Tyr185) antibody

多克隆 | SKU：bs-5487R

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货号:bs-3351R

￥1280

50μg

RMB1280

100μg

RMB1980

200μg

RMB2980

支持数据
产品信息
免疫原信息
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订购号:bs-5487R

￥1280.00-2900.00

50ul

100ul

200ul

货期：现货

售后保障

Rabbit Anti-phospho-MAPK8 ( Thr183 + Tyr185) antibody
产品应用（已验证）	IHC,FCM
产品应用（可尝试）	IF,ELISA
推荐稀释比例	Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=2ug/Test,IF=1:100-500,
研究领域	肿瘤,免疫学,信号转导,转录调节因子,激酶和磷酸酶
标签	Array

Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-MAPK8 ( Thr183 + Tyr185)) Polyclonal Antibody, Unconjugated (bs-5487R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Blank control: K562.
Primary Antibody (green line): Rabbit Anti-phospho-MAPK8 ( Thr183 + Tyr185) antibody (bs-5487R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-MAPK8 ( Thr183 + Tyr185)) Polyclonal Antibody, Unconjugated (bs-5487R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-MAPK8 ( Thr183 + Tyr185)) Polyclonal Antibody, Unconjugated (bs-5487R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

产品信息

RRID：AB_11086483
产品名称：Rabbit Anti-phospho-MAPK8 ( Thr183 + Tyr185) antibody
别名： MAPK8/MAPK9(phospho T183/Y185); JNK1+JNK2 (phospho T183 + Y185); AI849689; c Jun N terminal kinase 1; C-JUN kinase 1; c-Jun N-terminal kinase 1; EC 2.7.11.24; JNK 1; JNK; JNK-46; JNK1A2; JNK21B1/2; MAP kinase 8; MAPK 8; mapk8; Mitogen activat
中文名称：磷酸化氨基末端激酶2抗体
英文名称：Rabbit Anti-phospho-MAPK8 ( Thr183 + Tyr185) antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞核
性状：Liquid
亚型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

免疫原信息

免疫原：KLH conjugated Synthesised phosphopeptide derived from human MAPK9 around the phosphorylation site of Thr183+Tyr185
抗原表位：MM(p-T)P(p-Y)VV
SWISS：P45983
Gene ID ：5599
Human Gene ID：5599

产品介绍

phosphorylated at the Thr-Pro-Tyr phosphorylation motif instead of the characteristic MAP kinase Thr-Glu-Tyr motif. JNK2 (p54a, SAPK1a), along with JNK1 and JNK3, is thought to play an important role in nuclear signal transduction through its environmental stress activation and subsequent phosphorylation of the nuclear transcription factor p53.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.