| Rabbit Anti-phospho-CD130/gp130 (Ser782) antibody |
| 反应物种（预测） |
Mouse,Chicken,Dog,Pig,Cow,Horse,Rabbit |
| 产品应用（已验证） |
WB |
| 产品应用（可尝试） |
IHC,ICC,IF,ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100, |
| 研究领域 |
细胞生物,信号转导,细胞膜受体 |
| 标签 |
Array |
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Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-gp130 (Ser782)) Polyclonal Antibody, Unconjugated (bs-10122R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (Human cervical cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-gp130 (Ser782)) Polyclonal Antibody, Unconjugated (bs-10122R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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Sample: Raji (human)cell Lysate at 40 ug
Primary: Anti- p-gp130 (Ser782) (bs-10122R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 113kD
Observed band size: 123 kD
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Sample:
293T (human)cell Lysate at 40 ug
HepG2 (human)cell Lysate at 40 ug
Primary: Anti- p-gp130 (Ser782) (bs-10122R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 113kD
Observed band size: 123 kD
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phosphopeptide
non phosphopeptide
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Sample:
Lane 1: Human HepG2 cell Lysates
Lane 2: Human HUVEC cell Lysates
Primary: Anti-phospho-CD130gp130 (Ser782) (bs-10122R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 130kDa
Observed band size: 130kDa
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Sample:
Lane 1: HepG2 (Human) Cell Lysate at 30 ug
Lane 2: Hela (Human) Cell Lysate at 30 ug
Lane 3: A549 (Human) Cell Lysate at 30 ug
Lane 4: U87MG (Human) Cell Lysate at 30 ug
Primary: Anti-phospho-CD130/gp130 (Ser782) (bs-10122R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 130 kD
Observed band size: 140 kD
RRID：RRID
产品名称：Rabbit Anti-phospho-CD130/gp130 (Ser782) antibody
别名： gp130 (phospho S782); gp130 (phospho Ser782); p-gp130 (Ser782); Interleukin-6 receptor subunit beta; IL-6R-beta; Interleukin-6 signal transducer; Membrane glycoprotein 130; gp130; CDw130; Oncostatin-M receptor subunit alpha; CD_antigen; CD130; GP130 RAPS;
中文名称：磷酸化gp130抗体
英文名称：Rabbit Anti-phospho-CD130/gp130 (Ser782) antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞膜,分泌型蛋白
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthesised phosphopeptide derived from human gp130 around the phosphorylation site of Ser782
抗原表位：SE(p-S)TQ
SWISS：P40189
Gene ID ：3572
Human Gene ID：3572
CD130 is a signal transducer shared by many cytokines, including interleukin 6 (IL6), ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF), and oncostatin M (OSM). This protein functions as a part of the cytokine receptor complex. The activation of this protein is dependent upon the binding of cytokines to their receptors. vIL6, a protein related to IL6 and encoded by the Kaposi sarcoma-associated herpesvirus, can bypass the interleukin 6 receptor (IL6R) and directly activate this protein. Knockout studies in mice suggested a critical role of the gene encoding this protein in regulating myocyte apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been described.
Function：Signal-transducing molecule. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize gp130 for initiating signal transmission. Binds to IL6/IL6R (alpha chain) complex, resulting in the formation of high-affinity IL6 binding sites, an
Subunit：Interacts with INPP5D/SHIP1. Forms heterodimers composed of LIPR and IL6ST (type I OSM receptor). Also forms heterodimers composed of OSMR and IL6ST (type II OSM receptor). Homodimer. The homodimer binds two molecules of herpes virus IL6. Component of a h
Subcellular Location：Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted.
Tissue Specificity：Found in all the tissues and cell lines examined. Expression not restricted to IL6 responsive cells.
Post-translational modifications：Phosphorylation of Ser-782 down-regulates cell surface expression.
Heavily N-glycosylated.
Similarity：Belongs to the type I cytokine receptor family. Type 2 subfamily.
Contains 5 fibronectin type-III domains.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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