| Rabbit Anti-phospho-IGF1R (Tyr980) antibody |
| 反应物种（预测） |
Rat |
| 产品应用（已验证） |
IHC,FCM |
| 产品应用（可尝试） |
WB,IF,ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=1μg/Test,IF=1:100-500, |
| 研究领域 |
免疫学,生长因子和激素,转录调节因子,激酶和磷酸酶,细胞膜受体,糖尿病, |
| 标签 |
Array |
-
Blank control: MCF7.
Primary Antibody (green line): Rabbit Anti-phospho-IGF1R (Tyr980) antibody (bs-5447R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
-
The blue histogram is unstained cells(Hepg2 cells).
The green histogram is cells stained with Rabbit Anti-phospho-IGF1R (Tyr980) antibody (bs-5447R) plus secondary antibody（bs-0295G-FITC).Concentration 1:20
-
Tissue/cell: human kidney carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-phospho-IGF1R(Tyr980) Polyclonal Antibody, Unconjugated(bs-5447R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
-
Blank control(blue): Mouse Kidney (fixed with 2% paraformaldehyde for 10 min at 37℃).
Primary Antibody:Rabbit Anti-phospho-IGF1R (Tyr980) antibody (bs-5447R,Green); Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions;
Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
-
Paraformaldehyde-fixed, paraffin embedded (human stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RELM beta) Polyclonal Antibody, Unconjugated (bs-5774R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
RRID：AB_11109395
产品名称：Rabbit Anti-phospho-IGF1R (Tyr980) antibody
别名： IGF1 Receptor (phospho Y980); p-IGF1 Receptor (phospho Y980); IGF1R (phospho-Tyr980); IGF1R (phospho-Y980); p-IGF1R (Tyr980); p-IGF1R (Y980); IGF1R (phospho Tyr980); Insulin-like growth factor 1 receptor; CD221; CD221 antigen; IGF1 Receptor; IGF 1 recepto
中文名称：磷酸化胰岛素样生长因子1受体抗体
英文名称：Rabbit Anti-phospho-IGF1R (Tyr980) antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞膜
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated Synthesised phosphopeptide derived from human IGF1R around the phosphorylation site of Tyr980
抗原表位：PE(p-Y)FS
SWISS：P08069
Gene ID ：3480
Human Gene ID：3480
This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. [provided by RefSeq, Jul 2008].
Function：This receptor binds insulin-like growth factor 1 (IGF1) with a high affinity and IGF2 with a lower affinity. It has a tyrosine-protein kinase activity, which is necessary for the activation of the IGF1-stimulated downstream signaling cascade. When present
Subunit：Tetramer of 2 alpha and 2 beta chains linked by disulfide bonds. The alpha chains contribute to the formation of the ligand-binding domain, while the beta chain carries the kinase domain. Interacts with PIK3R1 and with the PTB/PID domains of IRS1 and SHC1
Subcellular Location：Membrane; Single-pass type I membrane protein.
Tissue Specificity：Found as a hybrid receptor with INSR in muscle, heart, kidney, adipose tissue, skeletal muscle, hepatoma, fibrobasts, spleen and placenta (at protein level). Expressed in a variety of tissues.
Post-translational modifications：The cytoplasmic domain of the beta subunit is autophosphorylated on tyrosine residues in response to insulin-like growth factor I (IGF I).
Phosphorylation of Tyr-980 is required for IRS1- and SHC1-binding.
DISEASE：Defects in IGF1R may be a cause in some cases of resistance to insulin-like growth factor 1 (IGF1 resistance) [MIM:270450]. IGF1 resistance is a gowth deficiency disorder characterized by intrauterine growth retardation and poor postnatal growth accompani
Similarity：Belongs to the protein kinase superfamily. Tyr protein kinase family.
Insulin receptor subfamily.
Contains 3 fibronectin type-III domains.
Contains 1 protein kinase domain.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
说明书
联系我们
打印此页面
收藏