Rabbit Anti-Phospho-TH (Ser31) antibody |
产品应用(已验证) |
WB,IHC |
产品应用(可尝试) |
IF,ELISA |
推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500, |
研究领域 |
肿瘤,细胞生物,免疫学,神经生物学,信号转导,内分泌病,新陈代谢,细胞类型标志物(Neuronal Marker), |
标签 |
Array |
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Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-TH (Ser31)) Polyclonal Antibody, Unconjugated (bs-3463R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-TH (Ser31)) Polyclonal Antibody, Unconjugated (bs-3463R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (Mouse adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-TH (Ser31)) Polyclonal Antibody, Unconjugated (bs-3463R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (Rat adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-TH (Ser31)) Polyclonal Antibody, Unconjugated (bs-3463R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
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Sample:
Lane 1: Adrenal gland (Mouse) Lysate at 40 ug
Lane 2: Adrenal gland (Rat) Lysate at 40 ug
Primary: Anti-Phospho-TH (Ser31) (bs-3463R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 59 kD
Observed band size: 59 kD
RRID:AB_10855294
产品名称:Rabbit Anti-Phospho-TH (Ser31) antibody
别名: Tyrosine Hydroxylase (Phospho-Ser31); Dystonia 14; DYT14; DYT5b; EC 1.14.16.2; OTTHUMP00000011225; OTTHUMP00000011226; ple; Protein Pale; TH; The; TY3H_RAT; TYH; Tyrosine 3 hydroxylase; Tyrosine 3 monooxygenase; Tyrosine 3-hydroxylase; Tyrosine 3-monooxyg
中文名称:磷酸化酪氨酸羟化酶抗体
英文名称:Rabbit Anti-Phospho-TH (Ser31) antibody
抗体来源: Rabbit
克隆类型:多克隆
细胞定位:细胞核,细胞浆,细胞膜
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原:KLH conjugated synthesised phosphopeptide derived from rat TH around the phosphorylation site of Ser31
抗原表位:VT(p-S)PR
SWISS:P04177
Gene ID :25085
Human Gene ID:7054
Tyrosine hydroxylase is involved in the conversion of tyrosine to dopamine. As the rate-limiting enzyme in the synthesis of catecholamines, tyrosine hydroxylase has a key role in the physiology of adrenergic neurons. Tyrosine hydroxylase is regularly used as a marker for dopaminergic neurons, which is particularly relevant for research into Parkinson's disease.
Function:Plays an important role in the physiology of adrenergic neurons.
Subunit:Homotetramer.
Subcellular Location:Mainly expressed in the brain and adrenal glands.
Post-translational modifications:In vitro, phosphorylation of Ser-19 increases the rate of Ser-40 phosphorylation, which results in enzyme opening and activation.
Similarity:Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.