| Rabbit Anti-phospho-Bcl-xL (Thr115) antibody |
| 反应物种（预测） |
Rat,Dog,Pig,Cow,Horse,Sheep |
| 产品应用（已验证） |
IHC |
| 产品应用（可尝试） |
WB,ICC,IF,ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100, |
| 研究领域 |
肿瘤,免疫学,信号转导,细胞凋亡,转录调节因子,新陈代谢, |
| 标签 |
Array |
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Independently Validated Antibody, image provided by Science Direct, badge number 029725:Formalin-fixed and paraffin embedded human prostate cancer labeled with Rabbit Anti-phospho-Bcl-xL (Thr115) Polyclonal Antibody, Unconjugated (bs-5234R) at 1:250 overnight at 4C followed by conjugation to the secondary antibody Envision Plus Horse Radish Peroxidase conjugated anti-rabbit antibody for two hours and DAB staining
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Independently Validated Antibody, image provided by Science Direct, badge number 029725:Formalin-fixed and paraffin embedded human ovary labeled with Rabbit Anti-phospho-Bcl-xL (Thr115) Polyclonal Antibody, Unconjugated (bs-5234R) at 1:250 overnight at 4C followed by conjugation to the secondary antibody and DAB staining
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Paraformaldehyde-fixed, paraffin embedded (mouse intestine tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Bcl-xL (Thr115)) Polyclonal Antibody, Unconjugated (bs-5234R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-Bcl-xL (Thr115)) Polyclonal Antibody, Unconjugated (bs-5234R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (mouset brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-Bcl-xL (Thr115)) Polyclonal Antibody, Unconjugated (bs-5234R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
RRID：AB_11064369
产品名称：Rabbit Anti-phospho-Bcl-xL (Thr115) antibody
别名： BCL2L1 (phospho T115); BCL2L1 (phospho Thr115); p-Bcl-xL(Thr115); p-BCL2L1(Thr115); Apoptosis regulator Bcl X; BclX; Bcl-X; Bcl 2 like 1; Bcl 2 like 1 protein; Bcl xL; BCL X/L; BCL XL/S; Bcl xS; Bcl2 like1; BCL2-related gene; BCL2-related protein, long is
中文名称：磷酸化Bcl-xL蛋白抗体
英文名称：Rabbit Anti-phospho-Bcl-xL (Thr115) antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞核,细胞浆,细胞膜
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated Synthesised phosphopeptide derived from human BCL2L1 around the phosphorylation site of Thr115
抗原表位：HI(p-T)PG
SWISS：Q07817
Gene ID ：598
Human Gene ID：598
The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The proteins encoded by this gene are located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Two alternatively spliced transcript variants, which encode distinct isoforms, have been reported. The longer isoform acts as an apoptotic inhibitor and the shorter form acts as an apoptotic activator. [provided by RefSeq, Jul 2008].
Function：Potent inhibitor of cell death. Inhibits activation of caspases (By similarity). Appears to regulate cell death by blocking the voltage-dependent anion channnel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mi
Subunit：Homodimer. Isoform Bcl-X(L) forms heterodimers with BAX, BAK or BCL2. Heterodimerization with BAX does not seem to be required for anti-apoptotic activity. Interacts with BCL2L11. Interacts with DMN1L; the interaction stimulates the GTPase activity of DMN
Subcellular Location：Mitochondrion membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein; Cytoplasmic side. Cytoplasm, cytoskeleton, centrosome. Note=Mitochondrial membranes and perinuclear envelope. Localizes to the centrosome when phosphoryl
Tissue Specificity：Bcl-X(S) is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. In contrast, Bcl-X(L) is found in tissues containing long-lived postmitotic cells, such as adult brain.
Post-translational modifications：Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity.
Phosphorylated on Ser-62 by CDK1. This phosphorylation is partial in normal mitotic cells, but complete in G2-arrested cells
Similarity：Belongs to the Bcl-2 family.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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