| Rabbit Anti-phospho-GluR1 (Ser645) antibody |
| 反应物种（预测） |
Rat,Chicken,Dog,Pig,Cow,Horse,Sheep |
| 产品应用（已验证） |
WB,IHC |
| 产品应用（可尝试） |
ICC,IF,ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500, |
| 研究领域 |
肿瘤,细胞生物,免疫学,神经生物学,信号转导,转录调节因子,细胞膜受体 |
| 标签 |
Array |
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Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-GluR1 (Ser645)) Polyclonal Antibody, Unconjugated (bs-11633R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Sample:
Lane 1: Human U-87 MG cell Lysates
Lane 2: Human U251 cell Lysates
Lane 3: Human SH-SY5Y cell Lysates
Primary: Anti-phospho-GluR1 (Ser645) (bs-11633R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 98kDa
Observed band size: 130kDa
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Sample:
Cerebrum (Mouse) Lysate at 40 ug
Cerebellum (Mouse) Lysate at 40 ug
Primary: Anti-phospho-GluR1 (Ser645) (bs-11633R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 98 kD
Observed band size: 128 kD
RRID：RRID
产品名称：Rabbit Anti-phospho-GluR1 (Ser645) antibody
别名： p-GluR-1(S645); p-GluR1(Ser645); Glutamate Receptor 1 (phospho S645); p-Glutamate Receptor 1 (phospho S645);;GLUR 1; GLUR A; AMPA 1; GluR-1; AMPA selective glutamate receptor 1; AMPA-selective glutamate receptor 1; GluA1; GLUH 1; GLUH1; GluR K1; GluR-1; G
中文名称：磷酸化谷氨酸受体1抗体
英文名称：Rabbit Anti-phospho-GluR1 (Ser645) antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞浆,细胞膜
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthesised phosphopeptide derived from human GluR1 around the phosphorylation site of Ser645
抗原表位：MV(p-S)PI
SWISS：P42261
Gene ID ：2890
Human Gene ID：2890
Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes with multiple subunits, each possessing transmembrane regions, and all arranged to form a ligand-gated ion channel. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. This gene belongs to a family of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008].
Function：Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation ch
Subunit：Homotetramer or heterotetramer of pore-forming glutamate receptor subunits. Tetramers may be formed by the dimerization of dimers. Interacts with DLG1 via its C-terminus. Interacts with SYNDIG1 and GRIA2. Interacts with LRFN. Interacts with HIP1 and RASGR
Subcellular Location：Cell membrane. Endoplasmic reticulum membrane.
Tissue Specificity：Widely expressed in brain.
Post-translational modifications：Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-603 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-829 palmitoylation does not affect cell surface expression but regulates stimulation-depende
Similarity：Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA1 subfamily.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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