| Rabbit Anti-phospho-CXCR4 (Ser339) antibody |
| 反应物种（预测） |
Human,Rat,Dog,Cow,Horse,Rabbit |
| 产品应用（已验证） |
WB,FCM |
| 产品应用（可尝试） |
ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,Flow Cyt=1μg/Test, |
| 研究领域 |
肿瘤,细胞生物,免疫学,神经生物学,干细胞,生长因子和激素,新陈代谢, |
| 标签 |
Array |
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Sample:
Lane 1: Lung (Mouse) Lysate at 40 ug
Lane 2: Spleen (Mouse) Lysate at 40 ug
Lane 3: Uterus (Mouse) Lysate at 40 ug
Lane 4: Lymph node (Mouse) Lysate at 40 ug
Primary:
Anti-phospho-CXCR4 (Ser339) (bs-12256R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kD
Observed band size: 50 kD
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Blank control(mouse):Mouse Spleen Cells (fixed with 2% paraformaldehyde for 10 min at 37℃).
Primary Antibody:Rabbit Anti-phospho-CXCR4 (Ser339) antibody (bs-12256R,Green); Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions;
Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
RRID：RRID
产品名称：Rabbit Anti-phospho-CXCR4 (Ser339) antibody
别名： p-CXCR4 (phospho S339); p-CD184 (phospho S339); C-X-C chemokine receptor type 4; CXC-R4; CXCR-4; Stromal cell-derived factor 1 receptor; SDF-1 receptor; Fusin; Leukocyte-derived seven transmembrane domain receptor; LESTR; CD184 antigen; CXCR4_HUMAN.
中文名称：磷酸化细胞表面趋化因子受体4抗体
英文名称：Rabbit Anti-phospho-CXCR4 (Ser339) antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞膜
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated Synthesised phosphopeptide derived from human CXCR4 around the phosphorylation site of Ser339
抗原表位：HS(p-S)VS
SWISS：P61073
Gene ID ：7852
Human Gene ID：7852
This gene encodes a CXC chemokine receptor specific for stromal cell-derived factor-1. The protein has 7 transmembrane regions and is located on the cell surface. It acts with the CD4 protein to support HIV entry into cells and is also highly expressed in breast cancer cells. Mutations in this gene have been associated with WHIM (warts, hypogammaglobulinemia, infections, and myelokathexis) syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. Monomer. Can form dimers.
Function：Receptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium ions levels and enhancing MAPK1/MAPK3 activation. Acts as a receptor for extracellular ubiquitin; leading to enhance intracellular calcium ions and
Subunit：Monomer. Can form dimmers.
Subcellular Location：Cell membrane; Multi-pass membrane protein.
Tissue Specificity：Expressed in numerous tissues, such as peripheral blood leukocytes, spleen, thymus, spinal cord, heart, placenta, lung, liver, skeletal muscle, kidney, pancreas, cerebellum, cerebral cortex and medulla (in microglia as well as in astrocytes), brain microv
Post-translational modifications：Phosphorylated on agonist stimulation. Rapidly phosphorylated on serine and threonine residues in the C-terminal. Phosphorylation at Ser-324 and Ser-325 leads to recruitment of ITCH, ubiquitination and protein degradation.
Ubiquitinated by ITCH at the
DISEASE：Defects in CXCR4 are a cause of WHIM syndrome (WHIM) [MIM:193670]; also known as warts, hypogammaglobulinemia, infections and myelokathexis. WHIM syndrome is an immunodeficiency disease characterized by neutropenia, hypogammaglobulinemia and extensive hum
Similarity：Belongs to the G-protein coupled receptor 1 family.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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