Rabbit Anti-C4orf21 antibody |
反应物种(预测) |
Rat,Cow,Horse |
产品应用(已验证) |
WB |
产品应用(可尝试) |
IHC,ICC,IF,ELISA |
推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500, |
研究领域 |
肿瘤,细胞生物,免疫学,发育生物学,表观遗传学, |
标签 |
Array |
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Tissue/cell: human laryngo carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-C4orf21 Polyclonal Antibody, Unconjugated(bs-15188R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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Sample:
Testis (Mouse) Lysate at 40 ug
Molt-4 (Human) Cell Lysate at 30 ug
HL60 (Human) Cell Lysate at 30 ug
Primary: Anti- C4orf21 (bs-15188R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 119 kD
Observed band size: 119 kD
RRID:RRID
产品名称:Rabbit Anti-C4orf21 antibody
别名: Chromosome 4 open reading frame 21; Uncharacterized protein C4orf21; Uncharacterized protein FLJ44066; YD002_HUMAN.
中文名称:4号染色体开放阅读框21抗体
英文名称:Rabbit Anti-C4orf21 antibody
抗体来源: Rabbit
克隆类型:多克隆
细胞定位:细胞膜
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原:KLH conjugated synthetic peptide derived from human C4orf21
抗原表位:101-200/926
SWISS:Q86YA3
Gene ID :55345
Human Gene ID:55345
C4orf12 (chromosome 4 open reading frame 12) is a 926 amino acid single-pass membrane protein that exists as two isoforms. The gene encoding C4orf21 maps to human chromosome 4. Human chromosome 4 represents approximately 6% of the human genome and contains nearly 900 genes. Notably, the Huntingtin gene, which is found to encode an expanded glutamine tract in cases of Huntington's disease, is on chromosome 4. FGFR-3 is also encoded by a gene that is located on chromosome 4, and has been associated with thanatophoric dwarfism, achondroplasia, Muenke syndrome and bladder cancer. Chromosome 4 is also tied to Ellis-van Creveld syndrome, methylmalonic acidemia and polycystic kidney disease.
Subcellular Location:Membrane; Single-pass membrane protein (Potential).
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.