| Rabbit Anti-phospho-Smad2/Smad3 (Thr8) antibody |
| 反应物种（预测） |
Rat,Chicken,Dog,Pig,Cow,Horse |
| 产品应用（已验证） |
WB,FCM |
| 产品应用（可尝试） |
IHC,ICC,IF,ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=1μg/Test,IF=1:100-500,ICC=1:100, |
| 研究领域 |
肿瘤,细胞生物,免疫学,信号转导,细胞凋亡,转录调节因子,表观遗传学, |
| 标签 |
Array |
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Sample:
HUVEC cell(human) Lysate at 30 ug
Primary: Anti- p-Smad2/Smad3 (Thr8) (bs-8853R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52kD
Observed band size: 52 kD
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Sample:
cerebrum(mouse) Lysate at 40 ug
RSC96 cell(rat) Lysate at 30 ug
hepG2 cell(human) Lysate at 30 ug
Hcclm3 cell(human) Lysate at 30 ug
Primary: Anti- p-Smad2/Smad3 (Thr8) (bs-8853R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52kD
Observed band size: 48,52 kD
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Sample:
U251 cell(human) Lysate at 30 ug
Primary: Anti- p-Smad2/Smad3 (Thr8) (bs-8853R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52kD
Observed band size: 52 kD
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blank: A549 cells (blue line)
isotype control: rabbit IgG (orange line)
second antibody: goat anti-rabbit IgG (white blue line)
primary antibody: rabbit Anti-phospho-Smad2/Smad3 (Thr8) (green line); contration: 3μg /10^6 cells
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Blank control: Hela.
Primary Antibody (green line): Rabbit Anti-phospho-Smad2/Smad3 (Thr8) antibody (bs-8853R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
RRID：RRID
产品名称：Rabbit Anti-phospho-Smad2/Smad3 (Thr8) antibody
别名： Smad2 + Smad3 (phospho T8); p-Smad2 + Smad3 (phospho T8);
P-Smad2 /3 (phospho T8);
hMAD 2;
hMAD 3;
hSMAD2;
hSMAD3;
Mad related protein 2;
MADH2;
MADH3;
MADR2;
Mothers against DPP homolog 2;
Mothers against DPP homolog 3;
Sma and Mad related pr
中文名称：磷酸化细胞信号转导分子SMAD2/SMAD3抗体
英文名称：Rabbit Anti-phospho-Smad2/Smad3 (Thr8) antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞核,细胞浆
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthesised phosphopeptide derived from human Smad2/Smad3 around the phosphorylation site of Thr8
抗原表位：PF(p-T)PP
SWISS：P84022
Gene ID ：4087
Human Gene ID：4087
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, May 2012]
Function：SMAD is a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathw
Subcellular Location：Cytoplasm. Nucleus. Note: Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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