Rabbit Anti-phospho-MEK2 (Thr394) antibody |
反应物种(预测) |
Human |
产品应用(已验证) |
WB,IHC |
产品应用(可尝试) |
IF,ELISA |
推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500, |
研究领域 |
肿瘤,免疫学,信号转导,转录调节因子,激酶和磷酸酶 |
标签 |
Array |
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Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-MEK2(Thr394)) Polyclonal Antibody, Unconjugated (bs-5427R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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Sample:
Lane 1: Mouse Brain Lysates
Lane 2: Mouse Pancrease Lysates
Lane 3: Rat Brain Lysates
Lane 4: Rat Pancrease Lysates
Primary: Anti-phospho-MEK2 (Thr394)(bs-5427R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 46kDa
Observed band size: 46 kDa
RRID:AB_11047634
产品名称:Rabbit Anti-phospho-MEK2 (Thr394) antibody
别名: MEK2 (phospho T394); p-MEK2 (phospho T394); MEK2(Phospho-Thr394); Cardiofaciocutaneous syndrome; CFC syndrome; Dual specificity mitogen activated protein kinase kinase 2; Dual specificity mitogen-activated protein kinase kinase 2; ERK activator kinase 2;
中文名称:磷酸化丝裂原活化蛋白激酶激酶2抗体
英文名称:Rabbit Anti-phospho-MEK2 (Thr394) antibody
抗体来源: Rabbit
克隆类型:多克隆
细胞定位:细胞浆,细胞膜
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原:KLH conjugated Synthesised phosphopeptide derived from human MEK2 around the phosphorylation site of Thr394
抗原表位:PG(p-T)PT
SWISS:P36507
Gene ID :5605
Human Gene ID:5605
The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. Mutations in this gene cause cardiofaciocutaneous syndrome (CFC syndrome), a disease characterized by heart defects, mental retardation, and distinctive facial features similar to those found in Noonan syndrome. The inhibition or degradation of this kinase is also found to be involved in the pathogenesis of Yersinia and anthrax. A pseudogene, which is located on chromosome 7, has been identified for this gene. [provided by RefSeq, Jul 2008].
Function:Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases.
Subunit:Interacts with MORG1 (By similarity). Interacts with SGK1.
Post-translational modifications:MAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1). Phosphorylated by MAP2K1/MEK1.
Acetylation of Ser-222 and Ser-226 by Yersinia yopJ prevents phosphorylation and activation, thus b
DISEASE:Defects in MAP2K2 are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio-cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defect
Similarity:Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
Contains 1 protein kinase domain.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.