Rabbit Anti-Integrin Alpha V + Beta 6 antibody |
产品应用(已验证) |
WB,FCM |
产品应用(可尝试) |
IHC,IF,ELISA |
推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=1μg/Test,IF=1:100-500, |
研究领域 |
肿瘤,心血管,细胞生物,免疫学,信号转导,生长因子和激素,细胞粘附分子,细胞骨架, |
标签 |
Array |
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Tissue/cell: human gastric carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Integrin Alpha V+Beta 6 Polyclonal Antibody, Unconjugated(bs-5791R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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Blank control(blue): Hela (fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice).
Primary Antibody:Rabbit Anti-Integrin Alpha V + Beta 6 antibody(bs-5791R), Dilution:1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
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Sample:
Lane 1: Kidney (Mouse) Lysate at 40 ug
Lane 2: U251 (Human) Cell Lysate at 30 ug
Lane 3: Huvec (Human) Cell Lysate at 30 ug
Primary: Anti-Integrin Alpha V + Beta 6 (bs-5791R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 125-140 kD
Observed band size: 120 kD
RRID:AB_11119310
产品名称:Rabbit Anti-Integrin Alpha V + Beta 6 antibody
别名: Integrin alpha 5 beta 6; CD51; Integrin alpha-V light chain; integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51); Integrin, alpha V; Integrin, beta 6; ITAV_HUMAN; ITGAV; ITGB6; Vitronectin receptor subunit alpha.
中文名称:整合素αVβ6抗体
英文名称:Rabbit Anti-Integrin Alpha V + Beta 6 antibody
抗体来源: Rabbit
克隆类型:多克隆
细胞定位:细胞膜
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原:KLH conjugated synthetic peptide derived from human Integrin Alpha V + Beta 6
SWISS:P06756
Gene ID :3685
Human Gene ID:3685
Integrins are heterodimeric cell surface receptors composed of alpha and beta subunits, which mediate cell-cell and cell-extracellular matrix attachments. Aberrant integrin expression has been found in many epithelial tumours. Changes in integrin expression have been shown to be important for the growth and early metastatic capacity of melanoma cells. Integrin alpha-v beta-6 is upregulated in cancers and during tissue remodelling but is absent from resting adult tissues. Integrin alpha-v beta-6 promotes invasion and correlates with poor survival and therefore makes a promising therapeutic target.
Function:The alpha-V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. In
Subunit:Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of an heavy and a light chain linked by a disulfide bond. Alpha-V associates with either beta-1, beta-3, beta-5, beta-6 or beta-8 subunit. Interacts with HIV-1 Tat. Alpha-V/beta-6 b
Subcellular Location:Membrane; Single-pass type I membrane protein.
Similarity:Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.