Rabbit Anti-MAPK organizer 1 antibody |
反应物种(预测) |
Human,Dog,Pig,Cow,Horse,Rabbit |
产品应用(已验证) |
WB,IHC |
产品应用(可尝试) |
IF,ELISA |
推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500, |
研究领域 |
心血管,细胞生物,信号转导,激酶和磷酸酶 |
标签 |
Array |
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Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MAPK organizer 1) Polyclonal Antibody, Unconjugated (bs-6722R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Sample:
Testis (Mouse) Lysate at 40 ug
Testis (Rat) Lysate at 40 ug
Primary: Anti-MAPK organizer 1 (bs-6722R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 26 kD
Observed band size: 26 kD
RRID:AB_11101629
产品名称:Rabbit Anti-MAPK organizer 1 antibody
别名: MAPK organizer 1; MGC4238; Mitogen activated protein kinase organizer 1; Mitogen-activated protein kinase organizer 1; MORG 1; WD repeat domain-containing protein 83; wdr83; WDR83_HUMAN.
中文名称:丝裂原活化蛋白激酶组织蛋白1抗体
英文名称:Rabbit Anti-MAPK organizer 1 antibody
抗体来源: Rabbit
克隆类型:多克隆
细胞定位:细胞核,细胞浆
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原:KLH conjugated synthetic peptide derived from human MAPK organizer 1
抗原表位:201-315/315
SWISS:Q9BRX9
Gene ID :84292
Human Gene ID:84292
This gene encodes a member of the WD-40 protein family. The protein is proposed to function as a molecular scaffold for various multimeric protein complexes. The protein associates with several components of the extracellular signal-regulated kinase (ERK) pathway, and promotes ERK activity in response to serum or other signals. The protein also interacts with egl nine homolog 3 (EGLN3, also known as PHD3) and regulates expression of hypoxia-inducible factor 1, and has been purified as part of the spliceosome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]
Function:Molecular scaffold protein for various multimeric protein complexes. Acts as a module in the assembly of a multicomponent scaffold for the ERK pathway, linking ERK responses to specific agonists. At low concentrations it enhances ERK activation, whereas h
Subunit:Interacts with EGLN3/PHD3. Interacts with ERK signaling proteins MAP2K1/MEK1, MAP2K2/MEK2, LAMTOR3, ARAF/Raf-1, MAPK1/ERK2 and MAPK3/ERK1. Identified in the spliceosome C complex.
Subcellular Location:Cytoplasm. Nucleus. Note=Predominantly cytoplasmic. Partially nuclear.
Similarity:Belongs to the WD repeat MORG1 family.
Contains 7 WD repeats.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.