Rabbit Anti-HOXC9 antibody |
反应物种(预测) |
Cow,Sheep |
产品应用(已验证) |
WB |
产品应用(可尝试) |
IHC,IF,ELISA |
推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500, |
研究领域 |
细胞生物,转录调节因子,表观遗传学, |
标签 |
Array |
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Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-HOXC9 Polyclonal Antibody, Unconjugated(bs-7982R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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Sample:
Lane 1: Muscle (Mouse) Tissue Lysate at 40 ug
Lane 2: Muscle (Rat) Tissue Lysate at 40 ug
Lane 3: Ear (Rat) Tissue Lysate at 40 ug
Primary: Anti-HOXC9 (bs-7982R) at 1/200 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 29 kD
Observed band size: 40 kD
RRID:RRID
产品名称:Rabbit Anti-HOXC9 antibody
别名: Homeo box C9; Homeobox C9; Homeobox protein Hox C9; Hox 3B; HOX3; HOX3B.
中文名称:同源盒蛋白HOXC9抗体
英文名称:Rabbit Anti-HOXC9 antibody
抗体来源: Rabbit
克隆类型:多克隆
细胞定位:细胞核
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原:KLH conjugated synthetic peptide derived from human HOXC9
抗原表位:151-220/260
SWISS:P31274
Gene ID :3225
Human Gene ID:3225
HOXC9 plays an important role in morphogenesis in all multicellular organisms. Mammals possess four similar homeobox gene clusters, HOXA, HOXB, HOXC and HOXD, which are located on different chromosomes and consist of 9 to 11 genes arranged in tandem.
Function:Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
Subcellular Location:Nucleus.
Similarity:Belongs to the Abd-B homeobox family.
Contains 1 homeobox DNA-binding domain.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.