Rabbit Anti-GPX4 antibody |
反应物种(预测) |
Pig,Cow |
产品应用(已验证) |
WB |
产品应用(可尝试) |
ELISA |
推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000, |
研究领域 |
肿瘤,细胞生物,免疫学,信号转导,新陈代谢,线抗体, |
标签 |
Array |
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Sample: Kidney (mouse) Lysate at 40 ug
Primary: Anti-GPX4 (bs-3884R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22kD
Observed band size: 20 kD
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Sample:
Lane 1: Testis (Mouse) Tissue Lysate at 40 ug
Lane 2: Liver (Mouse) Tissue Lysate at 40 ug
Lane 3: Kidney (Mouse) Tissue Lysate at 40 ug
Lane 4: Heart (Mouse) Tissue Lysate at 40 ug
Lane 5: Testis (Rat) Tissue Lysate at 40 ug
Lane 6: Liver (Rat) Tissue Lysate at 40 ug
Lane 7: Kidney (Rat) Tissue Lysate at 40 ug
Lane 8: Heart (Rat) Tissue Lysate at 40 ug
Lane 9: U937 (Human) Cell Lysate at 30 ug
Lane 10: HepG2 (Human) Cell Lysate at 30 ug
Lane 11: MCF-7 (Human) Cell Lysate at 30 ug
Lane 12: Huvec (Human) Cell Lysate at 30 ug
Lane 13: 293T (Human) Cell Lysate at 30 ug
Primary: Anti-GPX4 (bs-3884R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 20-22 kD
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Sample:
Testis (Mouse) Lysate at 40 ug
Primary: Anti-GPX4 (bs-3884R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 19 kD
RRID:AB_10857585
产品名称:Rabbit Anti-GPX4 antibody
别名: Glutathione peroxidase 4; GPX 4; GPX-4; GPX4; GPX4_HUMAN; GSHPx-4; MCSP; mitochondrial; PHGPx; Phospholipid hydroperoxidase; Phospholipid hydroperoxide glutathione peroxidase; Phospholipid hydroperoxide glutathione peroxidase; Phospholipid hydroperoxide g
中文名称:谷胱甘肽过氧化酶4抗体
英文名称:Rabbit Anti-GPX4 antibody
抗体来源: Rabbit
克隆类型:多克隆
细胞定位:细胞浆
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原:KLH conjugated synthetic peptide derived from human Glutathione Peroxidase 4
抗原表位:101-197/197
SWISS:P36969
Gene ID :2879
Human Gene ID:2879
Glutathione peroxidases (Gpxs) are ubiquitously expressed proteins which catalyze the reduction of hydrogen peroxides and organic hydroperoxides by glutathione. There are several isoforms which differ in their primary structure and localization. The classical cytosolic/mitochondrial GPx1 (cGPx) is a selenium-dependent enzyme, first of the GPx family to be discovered. GPx2, also known as gastrointestinal GPx (GI-GPx), is an intracellular enzyme expressed only at the epithelium of the gastrointestinal tract. Extracellular plasma GPx (pGPx or GPx3) is mainly expressed by the kidney from where it is released into the blood circulation. Phospholipid hydroperoxide GPx4 (PH-GPx) expressed in most tissues, can reduce many hydroperoxides including hydroperoxides integrated in membranes, hydroperoxy lipids in low density lipoprotein or thymine. All mammalian GPx family members, except for the recently described Cys containing GPx3 and epididymis-specific secretory GPx (eGPx or GPx5) isoforms, possess selenocysteine at the active site.
Function:Protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility. Could play a major role in protecting mammals from the toxicity of ingested lipid hydroperoxides. Essential for embryonic developm
Subunit:Monomer. Has a tendency to form higher mass oligomers.
Subcellular Location:Isoform Mitochondrial: Mitochondrion. Isoform Cytoplasmic: Cytoplasm.
Tissue Specificity:Present primarily in testis.
Similarity:Belongs to the glutathione peroxidase family.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.