Rabbit Anti-PNMT antibody |
反应物种(预测) |
Mouse,Dog,Pig,Horse,Rabbit |
产品应用(已验证) |
IHC,ICC |
产品应用(可尝试) |
IF,ELISA |
推荐稀释比例 |
Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100, |
研究领域 |
肿瘤,心血管,细胞生物,免疫学,神经生物学,转录调节因子 |
标签 |
Array |
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Paraformaldehyde-fixed, paraffin embedded (rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PNMT) Polyclonal Antibody, Unconjugated (bs-3912R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (rat adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PNMT) Polyclonal Antibody, Unconjugated (bs-3912R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (PNMT) polyclonal Antibody, Unconjugated (bs-3912R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
RRID:AB_10856728
产品名称:Rabbit Anti-PNMT antibody
别名: Noradrenaline N-methyltransferase; PENT; Phenylethanolamine N-methyltransferase; PNMTase; PNMT_HUMAN.
中文名称:苯乙醇胺甲基转移酶抗体
英文名称:Rabbit Anti-PNMT antibody
抗体来源: Rabbit
克隆类型:多克隆
细胞定位:细胞浆
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原:KLH conjugated synthetic peptide derived from human PNMT
抗原表位:101-200/282
SWISS:P11086
Gene ID :5409
Human Gene ID:5409
Phenylethanolamine N-methyltransferase, PNMT, catalyzes the synthesis of epinephrine from norepinephrine, (adrenalin from noradrenalin), in catecholamine biosynthesis.
Function:Converts noradrenaline to adrenaline.
Similarity:Belongs to the NNMT/PNMT/TEMT family.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.