| Rabbit Anti-FOG1 antibody |
| 反应物种（预测） |
Rat |
| 产品应用（已验证） |
WB |
| 产品应用（可尝试） |
IHC,IF,ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:50-200, |
| 研究领域 |
心血管,细胞生物,神经生物学,信号转导,锌指蛋白,表观遗传学, |
| 标签 |
Array |
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Tissue/cell: human esophageal carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-FOG1 Polyclonal Antibody, Unconjugated(bs-9451R) 1:400, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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Sample:
Cerebellum (Mouse) Lysate at 40 ug
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti- FOG1 (bs-9451R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 105 kD
Observed band size: 105 kD
RRID：RRID
产品名称：Rabbit Anti-FOG1 antibody
别名： FOG-1; FOG1_HUMAN; Friend of GATA 1; Friend of GATA protein 1; Friend of GATA1; ZFPM1; Zinc finger protein; Zinc finger protein 89A; Zinc finger protein multitype 1; Zinc finger protein ZFPM1.
中文名称：GATA结合蛋白1伴侣蛋白抗体
英文名称：Rabbit Anti-FOG1 antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞核
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human FOG1
抗原表位：1-100/1006
SWISS：Q8IX07
Gene ID ：161882
Human Gene ID：161882
Transcription regulator that plays an essential role in erythroid and megakaryocytic cell differentiation. Essential cofactor that acts via the formation of a heterodimer with transcription factors of the GATA family GATA1, GATA2 and GATA3. Such heterodimer can both activate or repress transcriptional activity, depending on the cell and promoter context. The heterodimer formed with GATA proteins is essential to activate expression of genes such as NFE2, ITGA2B, alpha- and beta-globin, while it represses expression of KLF1. May be involved in regulation of some genes in gonads. May also be involved in cardiac development, in a non-redundant way with ZFPM2/FOG2.
Function：Transcription regulator that plays an essential role in erythroid and megakaryocytic cell differentiation. Essential cofactor that acts via the formation of a heterodimer with transcription factors of the GATA family GATA1, GATA2 and GATA3. Such heterodim
Subunit：Interacts with corepressor CTBP2; this interaction is however not essential for corepressor activity (By similarity). Interacts with the N-terminal zinc-finger of GATA1, GATA2 and probably GATA3.
Subcellular Location：Nucleus.
Tissue Specificity：Mainly expressed in hematopoietic tissues. Also expressed in adult cerebellum, stomach, lymph node, liver and pancreas. Expressed in fetal heart, liver and spleen.
Similarity：Belongs to the FOG (Friend of GATA) family.
Contains 4 C2H2-type zinc fingers.
Contains 5 C2HC-type zinc fingers.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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