| Rabbit Anti-phospho-c-Jun (Thr91+Thr93) antibody |
| 反应物种（预测） |
Mouse,Rat,Chicken,Dog,Pig,Cow,Rabbit,Sheep |
| 产品应用（已验证） |
FCM |
| 产品应用（可尝试） |
ELISA |
| 推荐稀释比例 |
Elisa=1:5000-10000,Flow Cyt=1ug/Test, |
| 研究领域 |
肿瘤,染色质和核信号,信号转导,转录调节因子 |
| 标签 |
Array |
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Blank control: HepG2.
Primary Antibody (green line): Rabbit Anti-phospho-c-Jun (Thr91+Thr93) antibody (bs-1735R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
RRID：AB_10856243
产品名称：Rabbit Anti-phospho-c-Jun (Thr91+Thr93) antibody
别名： c-Jun (phospho T91 + T93); p-c-Jun(Thr91+Thr93); p-Jun(Thr91+Thr93); Transcription factor AP-1; Jun oncogene; JUN; AP 1; AP1; AP-1; Enhancer Binding Protein AP1; Jun Activation Domain Binding Protein; JUN protein; JUNC; p39; Proto oncogene cJun; Transcrip
中文名称：磷酸化原癌基因c-Jun抗体
英文名称：Rabbit Anti-phospho-c-Jun (Thr91+Thr93) antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞核
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated Synthesised phosphopeptide derived from human c-Jun around the phosphorylation site of Thr91/93
抗原表位：TT(p-T)P(p-T)PT
SWISS：P05412
Gene ID ：3725
Human Gene ID：3725
C-jun (Oncoprotein C-jun) is a component of the transcription factor AP-1 that binds and activates transcription at TRE/AP-1 elements and appears to be a major downstream target of the SAPK/JNK signaling pathway. The transcriptional activity of c-Jun is regulated by phosphorylation at Ser63 and Ser73. Extracellular signals including growth factors, transforming oncoproteins and UV irradiation stimulate phosphorylation of c-Jun at Ser63/73 and activate c-Jun dependent transcription. Mutation of Ser63/73 renders c-Jun nonresponsive to mitogenic and stress induced signaling pathways. The MAP kinase homologue, SAPK/JNK, binds to the N-terminal region of c-Jun and phosphorylates c-Jun at Ser63/73. In addition, the activity of SAPK/JNK is stimulated by the same signals that activate c-Jun.
Function：Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation.
Subunit：Heterodimer with either FOS or BATF3 or ATF7. The ATF7/JUN heterodimer is essential for ATF7 transactivation activity. Interacts with DSIPI; the interaction inhibits the binding of active AP1 to its target DNA (By similarity). Interacts with HIVEP3 and MY
Subcellular Location：Nucleus.
Post-translational modifications：Phosphorylated by CaMK4 and PRKDC; phosphorylation enhances the transcriptional activity. Phosphorylated by HIPK3. Phosphorylated by DYRK2 at Ser-243; this primes the protein for subsequent phosphorylation by GSK3B at Thr-239. Phosphorylated at Thr-239, S
Similarity：Belongs to the bZIP family. Jun subfamily.
Contains 1 bZIP (basic-leucine zipper) domain.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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