Rabbit Anti-ADAM32 antibody |
反应物种(预测) |
Rat,Dog,Cow,Horse,GuineaPig |
产品应用(已验证) |
WB |
产品应用(可尝试) |
IHC,IF,ELISA |
推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500, |
研究领域 |
肿瘤,细胞生物,免疫学,信号转导,激酶和磷酸酶,细胞骨架,细胞外基质,泛素, |
标签 |
Array |
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Sample:
U2os (Human) Cell Lysate at 30 ug
Hela(Human) Cell Lysate at 30 ug
Primary: Anti- ADAM32 (bs-5855R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 88 kD
Observed band size: 88 kD
RRID:AB_11109095
产品名称:Rabbit Anti-ADAM32 antibody
别名: A disintegrin and metalloproteinase domain 32; ADA32_HUMAN; ADAM 32; Adam32; Disintegrin and metalloproteinase domain-containing protein 32.
中文名称:去整合素样金属蛋白酶32抗体
英文名称:Rabbit Anti-ADAM32 antibody
抗体来源: Rabbit
克隆类型:多克隆
细胞定位:细胞膜
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原:KLH conjugated synthetic peptide derived from human ADAM32
抗原表位:181-280/787
抗原细胞定位:Extracellular
SWISS:Q8TC27
Gene ID :203102
Human Gene ID:203102
ADAM32 was first discovered in a search for testis-specific proteinases. ADAM32 was identified in human, rat, mouse, macaque and chimp, and thus far has been found only in testis. In mice, ADAM32 is found on the sperm surface, where it may play a role in fertilization. ADAM32 is a member of the ADAMs family (A Disintegrin And Metalloproteinase), but does not contain the canonical HExxHxxxxH zinc-binding metalloproteinase catalytic site. The domain structure of the full length ADAM32 includes a signal sequence, propeptide domain, metalloproteinase-like domain, disintegrin-like domain, cys-rich domain, EGF-like domain, a short spacer region, then the transmembrane domain and a cytoplasmic domain. Like many of the reproductive-specific ADAMS, ADAM32 plays a non-enzymatic role, or (as is the case for ADAMs 1 & 2 (fertilin alpha and beta)), the protein acts in concert with a proteolytically active ADAM to process proteins. Little is known about interactions between ADAM32 and other ADAMs. Several different sequences for human ADAM32 are published; 787, 688, 649, 629, and 279 amino acids in length. The 688 amino acid form is identical to the 787 AA form until the EGF-like domain, and lacks the TM and cytoplasmic domains. The 649 AA form is likewise identical to the longer form, just to the start of the TM domain, and also lacks the TM and cytoplasmic domains. The 629 AA form has a deletion of 107 residues midway into the MP-like domain, and lacks the amino end of the disintegrin domain, but contains the rest of the domains found in the full-length ADAM32. The predicted masses for the different versions are 87.8, 76.9, 72.9, 70.9 and 32.1, respectively, for the 786, 688, 649, 629 and 279 AA forms.
Function:May play a role in sperm development and fertilization This is a non-catalytic metalloprotease-like protein (By similarity)
Subcellular Location:Membrane; Single-pass type I membrane protein (Potential).
Tissue Specificity:Testis specific.
Similarity:Contains 1 disintegrin domain.
Contains 1 EGF-like domain.
Contains 1 peptidase M12B domain.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.