| Rabbit Anti-TUBB5 antibody |
| 反应物种（预测） |
Zebrafish,Sheep,Xenopus laevis |
| 产品应用（已验证） |
WB,IHC |
| 产品应用（可尝试） |
IF,ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500, |
| 研究领域 |
细胞类型标志物(Microtubule Marker),细胞骨架, |
| 标签 |
Array |
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Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TUBB5) Polyclonal Antibody, Unconjugated (bs-12863R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
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Sample:
Lane 1: Cerebellum (Rat) Lysate at 40 ug
Lane 2: Cerebellum (Mouse) Lysate at 40 ug
Lane 3: Cerebrum (Mouse) Lysate at 40 ug
Lane 4: Cerebrum (Rat) Lysate at 40 ug
Lane 5: Ovary (Mouse) Lysate at 40 ug
Lane 6: Heart (Mouse) Lysate at 40 ug
Lane 7: Heart (Rat) Lysate at 40 ug
Primary: Anti-TUBB5 (bs-12863R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 50 kD
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Sample: hela (human)cell Lysate at 40 ug
Primary: Anti- TUBB5(bs-12863R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 55 kD
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Sample: Eye (mouse) Lysate at 40 ug
Primary: Anti- TUBB5 (bs-12863R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 55 kD
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Sample: Bone (mouse) Lysate at 40 ug
Primary: Anti- TUBB5 (bs-12863R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 55 kD
RRID：RRID
产品名称：Rabbit Anti-TUBB5 antibody
别名： beta 4 tubulin; Beta 5 tubulin; beta Ib tubulin; M40; tubulin beta 1 chain; Tubulin beta 4 chain; tubulin beta 5 chain; Tubulin beta; Tubulin beta chain; tubulin beta polypeptide; TBB5_HUMAN.
中文名称：微管蛋白β5抗体
英文名称：Rabbit Anti-TUBB5 antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞浆
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human TUBB5
抗原表位：301-444/444
SWISS：P07437
Gene ID ：203068
Human Gene ID：203068
This gene encodes a beta tubulin protein. This protein forms a dimer with alpha tubulin and acts as a structural component of microtubules. Mutations in this gene cause cortical dysplasia, complex, with other brain malformations 6. Alternative splicing results in multiple splice variants. There are multiple pseudogenes for this gene on chromosomes 1, 6, 7, 8, 9, and 13. [provided by RefSeq, Jun 2014]
Function：Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Subunit：Dimer of alpha and beta chains. May interact with RNABP10. Interacts with PIFO. Interacts with MX1.
Subcellular Location：Cytoplasmic, cytoskeleton.
Tissue Specificity：Ubiquitously expressed with highest levels in spleen, thymus and immature brain.
Post-translational modifications：Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of fu
Similarity：Belongs to the tubulin family.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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