| Rabbit Anti-Phospho-HDAC3 (Ser424) antibody |
| 产品应用（已验证） |
WB,FCM |
| 产品应用（可尝试） |
IHC,IF,ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=1ug/Test,IF=1:100-500, |
| 研究领域 |
肿瘤,发育生物学,信号转导 |
| 标签 |
Array |
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Sample:
MCF-7(Human) Cell Lysate at 30 ug
NIH/3T3(Mouse) Cell Lysate at 30 ug
Hela(Human) Cell Lysate at 30 ug
Primary: Anti- Phospho-HDAC3 (Ser424) (bs-3174R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 50 kD
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Tissue/cell: Rat rectum tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Phospho-HDAC3, Unconjugated(bs-3174R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
RRID：AB_10857042
产品名称：Rabbit Anti-Phospho-HDAC3 (Ser424) antibody
别名： HDAC3 (phospho S424); HDAC3 (phospho Ser424); p-HDAC3 (Ser424); RPD3 2; RPD3-2; HD 3; HD3; HDAC 3; Histone deacetylase 3 (HD3) (RPD3-2); histone deacetylase 3; RPD 3; RPD3; SMAP 45; SMAP-45; SMAP45; HDAC3_HUMAN.
中文名称：磷酸化组蛋白去乙酰化酶3抗体
英文名称：Rabbit Anti-Phospho-HDAC3 (Ser424) antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞核,细胞浆
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated Synthesised phosphopeptide derived from human HDAC3 around the phosphorylation site of Ser424
抗原表位：KE(p-S)DV
SWISS：O15379
Gene ID ：8841
Human Gene ID：8841
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene. [provided by RefSeq, Jul 2008].
Function：Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4), and some other non-histone substrates. Histone deacetylation gives a tag for epigenetic repression and plays an important role in trans
Subunit：Interacts with HDAC7 and HDAC9. Forms a heterologous complex at least with YY1. Interacts with DAXX, HDAC10 and DACH1. Found in a complex with NCOR1 and NCOR2. Component of the N-Cor repressor complex, at least composed of NCOR1, NCOR2, HDAC3, TBL1X, TBL1
Subcellular Location：Nucleus.
Tissue Specificity：Widely expressed.
Post-translational modifications：Sumoylated in vitro.
Similarity：Belongs to the histone deacetylase family. HD type 1 subfamily.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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