Rabbit Anti-Phospho-Cyclin B1 (Ser133) antibody |
反应物种(预测) |
Mouse,Rat,Pig |
产品应用(已验证) |
ICC,FCM |
产品应用(可尝试) |
ELISA |
推荐稀释比例 |
Elisa=1:5000-10000,Flow Cyt=1μg /Test,ICC=1:100, |
研究领域 |
肿瘤 |
标签 |
Array |
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Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Phospho-Cyclin B1 (Ser133)) polyclonal Antibody, Unconjugated (bs-3123R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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Blank control (blue line): A549 (blue).
Primary Antibody (green line): Rabbit Anti-Phospho-Cyclin B1 (Ser133) antibody (bs-3123R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): F(ab’)2 fragment goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature.Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
RRID:AB_10857406
产品名称:Rabbit Anti-Phospho-Cyclin B1 (Ser133) antibody
别名: Cyclin B1 (phospho S133); Cyclin B1 (phospho-Ser133); p-Cyclin B1 (S133); p-Cyclin B1 (Ser133); p-Cyclin B1 (phospho S133); CCNB 1; CCNB; CCNB1; CCNB1_HUMAN; G2 mitotic specific cyclin B1; G2/mitotic-specific cyclin-B1.
中文名称:磷酸化周期素B1抗体
英文名称:Rabbit Anti-Phospho-Cyclin B1 (Ser133) antibody
抗体来源: Rabbit
克隆类型:多克隆
细胞定位:细胞核,细胞浆
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原:KLH conjugated Synthesised phosphopeptide derived from human Cyclin B1 around the phosphorylation site of Ser133
抗原表位:ET(p-S)GC
SWISS:P14635
Gene ID :891
Human Gene ID:891
The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq, Jul 2008].
Function:Essential for the control of the cell cycle at the G2/M (mitosis) transition.
Subunit:Interacts with the CDC2 protein kinase to form a serine/threonine kinase holoenzyme complex also known as maturation promoting factor (MPF). The cyclin subunit imparts substrate specificity to the complex. Binds HEI10. Interacts with catalytically active
Subcellular Location:Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, centrosome.
Post-translational modifications:Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases.
Phosphorylated by PLK1 at Ser-133 on centrosomes during prophase: phosphorylation by PLK1 does not cause nuclear import. Phosph
Similarity:Belongs to the cyclin family. Cyclin AB subfamily.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.