Rabbit Anti-Phospho-Vimentin (Ser56) antibody |
反应物种(预测) |
Rat,Dog,Pig,Cow,Sheep,GuineaPig |
产品应用(已验证) |
WB,FCM |
产品应用(可尝试) |
ELISA |
推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,Flow Cyt=0.2μg /Test, |
研究领域 |
肿瘤,细胞生物,免疫学,发育生物学,神经生物学,信号转导,干细胞,细胞骨架,肿瘤细胞生物标志物, |
标签 |
Array |
-
Sample:
Cerebrum(Mouse) Lysate at 40 ug
Kidney(Mouse) Lysate at 40 ug
Primary: Anti-Phospho-Vimentin (Ser56) (bs-3471R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54 kD
Observed band size: 53 kD
-
Sample:
Lane 1: Cerebrum (Mouse) Lysate at 40 ug
Lane 2: Kidney (Mouse) Lysate at 40 ug
Lane 3: Hela (Human) Cell Lysate at 30 ug
Lane 4: NIH/3T3 (Mouse) Cell Lysate at 30 ug
Lane 5: Spleen (Mouse) Lysate at 40 ug
Lane 6: Lymph node (Mouse) Lysate at 40 ug
Lane 7: Testis (Mouse) Lysate at 40 ug
Lane 8: SH-SY5Y (Human) Cell Lysate at 30 ug
Lane 9: A549 (Human) Cell Lysate at 30 ug
Primary: Anti-Phospho-Vimentin (Ser56) (bs-3471R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 51 kD
-
Blank control (blue line): Hela (fixed with 80% methanol (5 min at -20℃) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature).
Primary Antibody (green line): Rabbit Anti- Phospho-Vimentin (Ser56) antibody (bs-3471R),Dilution: 0.2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC,Dilution: 1μg /test.
RRID:AB_10853529
产品名称:Rabbit Anti-Phospho-Vimentin (Ser56) antibody
别名: Vimentin (phospho S56); p-Vimentin (phospho S56); FLJ36605; OTTHUMP00000019224; VIM; VIME_HUMAN; Vimentin.
中文名称:磷酸化波形蛋白抗体
英文名称:Rabbit Anti-Phospho-Vimentin (Ser56) antibody
抗体来源: Rabbit
克隆类型:多克隆
细胞定位:细胞浆
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原:KLH conjugated Synthesised phosphopeptide derived from human Vimentin around the phosphorylation site of Ser56
抗原表位:S(p-S)PG
SWISS:P08670
Gene ID :7431
Human Gene ID:7431
Vimentin is the major subunit protein of the intermediate filaments of mesenchymal cells. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain-dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis. Immunohistochemical staining for Vimentin is characteristic of sarcomas (of neural, muscle and fibroblast origin) compared with carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin. Vimentin antibodies are thus of value in the differential diagnosis of undifferentiated neoplasms and malignant tumors. They are generally used with a panel of other antibodies including those recognizing cytokeratins, lymphoid markers, S100, desmin and neurofilaments.
Function:Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Involved with LARP6 in the
Subunit:Homopolymer. Interacts with HCV core protein. Interacts with LGSN and SYNM. Interacts (via rod region) with PLEC (via CH 1 domain).Interacts with SLC6A4. Interacts with STK33. Interacts with LARP6. Interacts with RAB8B.
Subcellular Location:Cytoplasm.
Tissue Specificity:Highly expressed in fibroblasts, somev expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
Post-translational modifications:Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which
Similarity:Belongs to the intermediate filament family.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.