Rabbit Anti-GCNT1 antibody,蛋白Marker-蛋白检测试剂盒-IVD原料-北京博奥森生物技术有限公司

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Rabbit Anti-GCNT1 antibody

多克隆 | SKU：bs-12334R

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货号:bs-3351R

￥1280

50μg

RMB1280

100μg

RMB1980

200μg

RMB2980

支持数据
产品信息
免疫原信息
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订购号:bs-12334R

￥1138.00-2900.00

50ul

100ul

200ul

货期：现货

售后保障

Rabbit Anti-GCNT1 antibody
反应物种（预测）	Dog,Cow,Sheep
产品应用（已验证）	WB,IHC
产品应用（可尝试）	ICC,IF,ELISA
推荐稀释比例	WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500,
研究领域	肿瘤,细胞生物,信号转导,新陈代谢,糖蛋白,
标签	Array

Paraformaldehyde-fixed, paraffin embedded (human pancreatic cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GCNT1) Polyclonal Antibody, Unconjugated (bs-12334R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Sample:
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti- GCNT1 (bs-12334R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 50 kD
Sample:
U87MG (Human) Cell Lysate at 30 ug
Primary: Anti- GCNT1 (bs-12334R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 52 kD
Sample:
Lane 1: Rat Lymph node tissue lysates
Lane 2: Human SW480 cell lysates
Lane 3: Human TT cell lysates
Lane 4: Human U87MG cell lysates
Lane 5: Human MCF-7 cell lysates
Primary: Anti-GCNT1 (bs-12334R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 50 kD

产品信息

RRID：RRID
产品名称：Rabbit Anti-GCNT1 antibody
别名： 3-galactosyl-O-glycosyl-glycoprotein beta-1; 6-N-acetylglucosaminyltransferase; Beta-1; Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase; C2GNT; C2GNT L; C2GNT1; C2GnTL; Core 2 branching enzyme; Core 2 GNT; Core 2-branc
中文名称：GCNT1葡萄糖转移酶抗体
英文名称：Rabbit Anti-GCNT1 antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞浆,细胞膜
性状：Liquid
亚型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

免疫原信息

免疫原：KLH conjugated synthetic peptide derived from Human GCNT1
抗原表位：201-260/428
SWISS：Q02742
Gene ID ：2650
Human Gene ID：2650

产品介绍

GCNT1 is expressed in a variety of cell types, including lymphocytes and mucin-producing cells. Specifically, GCNT1 expression in leukocytes regulates the synthesis of core 2 O-glycans that carry sialyl-Lewis x (sLex) oligosaccharides, which confer high affinity binding to Selectins. Downregulation of Selectin ligand expression levels has been shown to inhibit tissue infiltration. Therefore, GCNT1 represents a potential drug target for the treatment of inflammatory disorders and other pathologies involving Selectins.
Function：Forms critical branches in O-glycans.
Subcellular Location：Golgi apparatus membrane.
Tissue Specificity：Highly expressed in activated T-lymphocytes and myeloid cells.
Similarity：Belongs to the glycosyltransferase 14 family.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.