| Rabbit Anti-COLGALT1 antibody |
| 反应物种（预测） |
Dog,Pig,Cow,Horse,Sheep |
| 产品应用（已验证） |
WB,FCM |
| 产品应用（可尝试） |
ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,Flow Cyt=2ug/Test, |
| 研究领域 |
细胞生物,信号转导,新陈代谢,细胞外基质, |
| 标签 |
Array |
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Blank control:Mouse spleen.
Primary Antibody (green line): Rabbit Anti-GLT25D1 antibody (bs-13378R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 1μg /test.
Protocol
The cells were fixed with 70% ethanol (10min at room temperature) and then were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
RRID：RRID
产品名称：Rabbit Anti-COLGALT1 antibody
别名： GLT25D1; FLJ22329; Glycosyltransferase 25 domain containing 1; Hydroxylysine galactosyltransferase 1; Procollagen galactosyltransferase 1; GT251_HUMAN.
中文名称：糖基转移酶25结构域1/前胶原半乳糖基转移酶1抗体
英文名称：Rabbit Anti-COLGALT1 antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞浆
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human GLT25D1
抗原表位：531-622/622
SWISS：Q8NBJ5
Gene ID ：79709
Human Gene ID：79709
GLT25D1 is a 622 amino acid protein that localizes to the lumen of the endoplasmic reticulum. Ubiquitously expressed with higher levels in placenta, heart, lung and spleen, GLT25D1 is a member of the glycosyltransferase 25 family. The beta-galactosyltransferase activity of GLT25D1 allows the transfer of beta-galactose to hydroxylysine residues of collagen. GLT25D1 is considered an important target for investigating the biological significance of collagen glycosylation and the importance of posttranslational modification in the etiology of connective tissue disorders. The gene encoding GLT25D1 is located on human chromosome 19, which consists of over 63 million bases, houses approximately 1,400 genes and is recognized for having the greatest gene density of the human chromosomes.
Function：Has a beta-galactosyltransferase activity; transfers beta-galactose to hydroxylysine residues of collagen.
Subcellular Location：Endoplasmic reticulum lumen. Note: Colocalized with PLOD3 and mannose binding lectin.
Tissue Specificity：Ubiquitous with higher levels in placenta, heart, lung and spleen.
Post-translational modifications：N-glycosylated.
Similarity：Belongs to the glycosyltransferase 25 family.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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