Rabbit Anti-phospho-IRS1 (Ser616) antibody,蛋白Marker-蛋白检测试剂盒-IVD原料-北京博奥森生物技术有限公司

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磷酸化抗体

Rabbit Anti-phospho-IRS1 (Ser616) antibody

多克隆 | SKU：bs-12710R

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货号:bs-3351R

￥1280

50μg

RMB1280

100μg

RMB1980

200μg

RMB2980

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产品信息
免疫原信息
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订购号:bs-12710R

￥1280.00-2900.00

50ul

100ul

200ul

货期：现货

售后保障

Rabbit Anti-phospho-IRS1 (Ser616) antibody
反应物种（预测）	Chicken,Dog,Pig,Cow,Horse,Rabbit,Sheep
产品应用（已验证）	WB,IHC,ICC,FCM
产品应用（可尝试）	IF,ELISA
推荐稀释比例	WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=1μg/Test,IF=1:100-500,ICC=1:100,
研究领域	细胞生物,免疫学,神经生物学,细胞凋亡,糖尿病,细胞类型标志物,
标签	Array

Blank control: HepG2.
Primary Antibody (green line): Rabbit Anti-phospho-IRS1 (Ser616) antibody (bs-12710R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Paraformaldehyde-fixed, paraffin embedded (rat uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-IRS1 (Ser616)) Polyclonal Antibody, Unconjugated (bs-12710R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Sample:
HepG2(Human) Cell Lysate at 30 ug
Primary: Anti-phospho-IRS1 (Ser616) (bs-12710R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 131 kD
Observed band size: 131 kD
Sample:
MG63(Human) Cell Lysate at 30 ug
Primary: Anti-phospho-IRS1 (Ser616) (bs-12710R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 131 kD
Observed band size: 131 kD
A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (phospho-IRS1 (Ser616)) polyclonal Antibody, Unconjugated (bs-12710R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Blank control (blue line): Hela (fixed with 70% ethanol (Overnight at 4℃) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature).
Primary Antibody (green line): Rabbit Anti-phospho-IRS1 (Ser616) antibody (bs-12710R),Dilution: 0.2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE,Dilution: 1μg /test.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-IRS1 (Ser616)) Polyclonal Antibody, Unconjugated (bs-12710R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Sample: MCF-7 Cell (Human) Lysate at 40 ug
Primary: Anti-phospho-IRS1 (Ser616) (bs-12710R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 131 kD
Observed band size: 131 kD
Sample: MCF-7 Cell (Human) Lysate at 40 ug
Primary: Anti-phospho-IRS1 (Ser616) (bs-12710R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 131 kD
Observed band size: 131 kD

产品信息

RRID：RRID
产品名称：Rabbit Anti-phospho-IRS1 (Ser616) antibody
别名： IRS1 (phospho S616); p-IRS1 (phospho S616); IRS1_HUMAN; Insulin receptor substrate 1; IRS-1; IRS 1;
中文名称：磷酸化胰岛素受体底物1抗体
英文名称：Rabbit Anti-phospho-IRS1 (Ser616) antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞核,细胞浆,细胞膜
性状：Liquid
亚型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

免疫原信息

免疫原：KLH conjugated synthesised phosphopeptide derived from human IRS1 around the phosphorylation site of Ser616
抗原表位：PM(p-S)PG
SWISS：P35568
Gene ID ：3667
Human Gene ID：3667

产品介绍

This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance. [provided by RefSeq, Nov 2009]
Function：May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosp
Subunit：Interacts with UBTF and PIK3CA (By similarity). Interacts (via phosphorylated YXXM motifs) with PIK3R1 (By similarity). Interacts with ROCK1 and FER (By similarity). Interacts (via PH domain) with PHIP (By similarity). Interacts with GRB2 (By similarity).
Post-translational modifications：Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor. Phosphorylation of Tyr-896 is required for GRB2-binding.
DISEASE：Polymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853].
Similarity：Contains 1 IRS-type PTB domain.
Contains 1 PH domain.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.