Rabbit Anti-phospho-Hsp90 beta (Ser226) antibody,蛋白Marker-蛋白检测试剂盒-IVD原料-北京博奥森生物技术有限公司

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磷酸化抗体

Rabbit Anti-phospho-Hsp90 beta (Ser226) antibody

多克隆 | SKU：bs-18074R

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货号:bs-3351R

￥1280

50μg

RMB1280

100μg

RMB1980

200μg

RMB2980

支持数据
产品信息
免疫原信息
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订购号:bs-18074R

￥1280.00-2900.00

50ul

100ul

200ul

货期：现货

售后保障

Rabbit Anti-phospho-Hsp90 beta (Ser226) antibody
反应物种（预测）	Mouse,Rat
产品应用（已验证）	FCM
产品应用（可尝试）	ELISA
推荐稀释比例	Elisa=1:5000-10000,Flow Cyt=1μg/Test,
研究领域	细胞生物,信号转导
标签	Array

Blank control (blue line): A549 (fixed with 70% ethanol (Overnight at 4℃) and then permeabilized with 90% ice-cold methanol for 30 min on ice).
Primary Antibody (green line): Rabbit Anti-phospho-Hsp90 beta (Ser226)antibody (bs-18074R),Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC,Dilution: 1μg /test.
U2OS cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (phospho-Hsp90 beta (Ser226)) polyclonal Antibody, Unconjugated (bs-18074R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Blank control:Hela.
Primary Antibody (green line): Rabbit Anti-phospho-Hsp90 beta (Ser226) antibody (bs-18074R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1%PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

产品信息

RRID：RRID
产品名称：Rabbit Anti-phospho-Hsp90 beta (Ser226) antibody
别名： Hsp90 beta (phospho S226); p-Hsp90 beta (phospho S226); 90 kda heat shock protein beta HSP90 beta; D6S182; FLJ26984; Heat shock 84 kDa; Heat shock 90kD protein 1, beta; Heat shock 90kDa protein 1 beta; Heat shock protein 90kDa alpha (cytosolic) clas
中文名称：磷酸化热休克蛋白90β抗体
英文名称：Rabbit Anti-phospho-Hsp90 beta (Ser226) antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞浆
性状：Liquid
亚型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

免疫原信息

免疫原：KLH conjugated synthesised phosphopeptide derived from human Hsp90 beta around the phosphorylation site of Ser226
抗原表位：EI(p-S)DD
SWISS：P08238
Gene ID ：3326
Human Gene ID：3326

产品介绍

This gene encodes a member of the heat shock protein 90 family; these proteins are involved in signal transduction, protein folding and degradation and morphological evolution. This gene encodes the constitutive form of the cytosolic 90 kDa heat-shock protein and is thought to play a role in gastric apoptosis and inflammation. Alternative splicing results in multiple transcript variants. Pseudogenes have been identified on multiple chromosomes. [provided by RefSeq, Dec 2012]
Function：Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activ
Subcellular Location：Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Post-translational modifications：Ubiquitinated in the presence of STUB1-UBE2D1 complex (in vitro).
ISGylated.
S-nitrosylated; negatively regulates the ATPase activity.
Similarity：Belongs to the heat shock protein 90 family.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.