Rabbit Anti-SLC25A11 antibody |
反应物种(预测) |
Rat,Cow,Rabbit,Sheep,GuineaPig |
产品应用(已验证) |
WB,FCM |
产品应用(可尝试) |
ELISA |
推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,Flow Cyt=1ug/Test, |
研究领域 |
肿瘤,细胞生物,信号转导,转运蛋白, |
标签 |
Array |
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Sample:
Liver (Mouse) Lysate at 40 ug
Primary: Anti-SLC25A15 (bs-8703R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kD
Observed band size: 34 kD
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Blank control: THP-1.
Primary Antibody (green line): Rabbit Anti-SLC25A15 antibody (bs-8703R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST methanol for 20 min at room temperature.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature.The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
RRID:RRID
产品名称:Rabbit Anti-SLC25A11 antibody
别名: M2OM_HUMAN;
Mitochondrial 2 oxoglutarate/malate carrier protein;
Mitochondrial 2-oxoglutarate/malate carrier protein;
OGC;
OGCP;
SLC20A4;
Solute carrier family 20 (oxoglutarate carrier) member 4;
Solute carrier family 20 member 4;
Solute carrier
中文名称:溶质载体家族25成员11抗体
英文名称:Rabbit Anti-SLC25A11 antibody
抗体来源: Rabbit
克隆类型:多克隆
细胞定位:细胞膜
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原:KLH conjugated synthetic peptide derived from human SLC25A15
抗原表位:101-200/314
SWISS:Q02978
Gene ID :8402
Human Gene ID:8402
The oxoglutarate/malate carrier transports 2-oxoglutarate across the inner membranes of mitochondria in an electroneutral exchange for malate or other dicarboxylic acids (summary by Iacobazzi et al., 1992 [PubMed 1457818]).[supplied by OMIM, Jan 2011]
Function:Catalyzes the transport of 2-oxoglutarate across the inner mitochondrial membrane in an electroneutral exchange for malate or other dicarboxylic acids, and plays an important role in several metabolic processes, including the malate-aspartate shuttle, the
Subcellular Location:Mitochondrion inner membrane.
Similarity:Belongs to the mitochondrial carrier family.
Contains 3 Solcar repeats.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.