Rabbit Anti-Phospho-CDK1 (Ser39) antibody |
反应物种(预测) |
Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit,Sheep |
产品应用(已验证) |
WB |
产品应用(可尝试) |
ELISA |
推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000, |
研究领域 |
肿瘤,免疫学,信号转导,细胞凋亡,细胞周期蛋白,转录调节因子 |
标签 |
Array |
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Sample:
A431(Human) Cell Lysate at 30 ug
Primary: Anti-Phospho-CDK1 (Ser39) (bs-5247R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kD
Observed band size: 34 kD
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Sample:
Spleen (Mouse) Lysate at 40 ug
A549(Human) Cell Lysate at 30 ug
Uterus(Mouse) Lysate at 40 ug
Primary: Anti- Phospho-CDK1 (Ser39) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kD
Observed band size: 34 kD
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Sample:
A431(Human) Cell Lysate at 30 ug
Thymus (Mouse) Lysate at 40 ug
Primary: Anti- Phospho-CDK1 (Ser39) (bs-5247R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kD
Observed band size: 34 kD
RRID:AB_11078169
产品名称:Rabbit Anti-Phospho-CDK1 (Ser39) antibody
别名: P34CDC2; Cdc 2; cdc2; CDK 1; CDK1; Cell division control protein 2; Cell division control protein 2 homolog; Cell division cycle 2 G1 to S and G2 to M; Cell Divsion Cycle 2 Protein; CDK1_HUMAN.
中文名称:磷酸化周期素依赖激酶2抗体
英文名称:Rabbit Anti-Phospho-CDK1 (Ser39) antibody
抗体来源: Rabbit
克隆类型:多克隆
细胞定位:细胞核,细胞浆
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原:KLH conjugated Synthesised phosphopeptide derived from human cdc2 around the phosphorylation site of Ser39
抗原表位:LE(p-S)EE
SWISS:P06493
Gene ID :983
Human Gene ID:983
The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Function:Plays a key role in the control of the eukaryotic cell cycle by modulating the centrosome cycle as well as mitotic onset; promotes G2-M transition, and regulates G1 progress and G1-S transition via association with multiple interphase cyclins. Required in
Subunit:Forms a stable but non-covalent complex with a regulatory subunit and with a cyclin. Interacts with cyclins-B (CCNB1, CCNB2 and CCNB3) to form a serine/threonine kinase holoenzyme complex also known as maturation promoting factor (MPF). The cyclin subunit
Subcellular Location:Nucleus. Cytoplasm. Mitochondrion. Cytoplasm, cytoskeleton, centrosome. Note=Cytoplasmic during the interphase. Reversibly translocated from cytoplasm to nucleus when phosphorylated before G2-M transition when associated with cyclin-B1. Accumulates in mit
Tissue Specificity:Isoform 2 is found in breast cancer tissues.
Post-translational modifications:Phosphorylation at Thr-161 by CAK/CDK7 activates kinase activity. Phosphorylation at Thr-14 and Tyr-15 by PKMYT1 prevents nuclear translocation. Phosphorylation at Tyr-15 by WEE1 and WEE2 inhibits the protein kinase activity and acts as a negative regulat
Similarity:Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.